Lignosulfonate is a by-product of the cooking process by sulfite pulping for paper manufacturing. The treatment of wood chips by various salts of sulfurous acid solubilizes lignin to produce a cellulose-rich wood pulp. Developing a technique for the conversion of lignosulfonate by-product to high value materials has an important industrial utility. Sphingobium sp. strain SYK-6, which was isolated from pulping wastewater, is one of the best enzymatically or genetically characterized bacteria for degrading lignin-derived aromatics. We have previously established a system for the production of 2-pyrone-4,6-dicarboxylic acid (PDC), a novel platform chemical that can produce a variety of bio-based polymers, by introducing of ligA, ligB, and ligC genes from SYK-6 into a mutant strain of Pseudomonas putida PpY1100. In this study, extracts from lignosulfonates, which were desulphonated and depolymerized by alkaline oxidation, were evaluated as substrates for microbiological conversion to PDC by the transgenic bacteria.

木质素磺酸盐是亚硫酸盐制浆造纸过程中蒸煮过程的副产品。用各种亚硫酸盐处理木片会使木质素溶解，从而生产出富含纤维素的木浆。开发将木质素磺酸盐副产物转化为高价值材料的技术具有重要的工业实用性。鞘氨醇单胞菌 从制浆废水中分离出的SYK-6菌株是用于降解木质素衍生的芳烃的最好的酶或遗传特征细菌之一。我们之前已经建立了用于生产2-pyrone-4,6-dihydroxy（PDC）的系统，这是一种新型平台化学品，可通过引入ligA，ligB和ligC来生产各种基于生物的聚合物基因从SYK-6转移到恶臭假单胞菌PpY1100的突变株中。在这项研究中，木质素磺酸盐的提取物经碱氧化脱硫和解聚后，被评估为转基因细菌微生物转化为PDC的底物。