Macrolactins (MLNs) have attracted considerable attention due to their antibacterial and antiviral properties. Here, the MLN production of Bacillus sp. strain IMDGX0108 was improved using a breeding strategy of atmospheric room temperature plasma (ARTP) technique. Combining with a selection procedure based on the colony morphology and specific growth rate index (SGRI), two genetically stable mutants A29 and A72 were identified. The MLN production of A29 and A72 was 35.2% and 52.8% greater than that of the parent strain, respectively. The best-performing mutant A72 was subjected to RNA-sequence analysis. Five pathways were significantly enriched, and fatty acid bioprocesses might play an important role in improving the production of MLNs. The combined strategy developed herein (i.e., ARTP mutation plus an efficient screening procedure) might be an appropriate method by which to obtain strains overproducing MLNs.

巨乳素（MLNs）由于其抗菌和抗病毒特性而引起了广泛的关注。在这里，MLN产生的芽孢杆菌sp。IMDGX0108菌株使用大气室温等离子体（ARTP）技术的育种策略进行了改良。结合基于菌落形态和比生长速率指数（SGRI）的选择程序，鉴定了两个遗传稳定的突变体A29和A72。A29和A72的MLN产量分别比亲本菌株高35.2％和52.8％。对表现最佳的突变体A72进行RNA序列分析。五个途径得到了丰富，脂肪酸的生物过程可能在提高MLN的产生中起重要作用。本文开发的组合策略（即ARTP突变加上有效的筛选程序）可能是获得过量生产MLN菌株的合适方法。