High temperature fermentation can substantially reduce the contaminations and condensation costs. Candida glycerinogenes was more resistant to high temperature than Saccharomyces cerevisiae. Quantitative real-time PCR results showed that 7 of 33 candidates served as potential high temperature inducible promoters in C. glycerinogenes. Fluorescence analysis indicated that PCgcwp1 showed the highest activity at 42°C. PCgaac and PCgpot1 showed medium–high expression, while PCghsp12, PCgatp1, PCgino1 and PCgscl were modest or weaker expression. Above seven promoters were further used to control the expression of xylose reductase gene from Neurospora crassa in C. glycerinogenes. Compared with 30°C, the xylitol yields of the seven recombinant strains were all improved at 42°C, and PCgcwp1 showed the highest xylitol production which was increased by 204% and reached 15.4 g/L. These results showed a potential to high temperature fermentation on the stress tolerant C. glycerinogenes and some of novel high temperature inducible promoters.

高温发酵可以大大减少污染和冷凝成本。假丝酵母念珠菌比酿酒酵母更耐高温。实时定量PCR结果显示，33个候选基因中有7个是甘油三酸杆菌基因中潜在的高温诱导型启动子。荧光分析表明，P Cgcwp1在42°C时显示最高活性。P Cgaac和P Cgpot1显示中等至高表达，而P Cghsp12，P Cgatp1，P Cgino1和P Cgsc​​l表情适中或较弱。以上7个启动子进一步用于控制毛孢神经霉中木糖还原酶基因在C.甘油基因中的表达。与30°C相比，七种重组菌株的木糖醇收率均在42°C时有所提高，而P Cgcwp1的木糖醇产量最高，增加了204％，达到15.4 g / L。这些结果表明在耐胁迫的甘油三酸甘油酯基因和一些新型的高温诱导型启动子上进行高温发酵的潜力。