Abstract Background Long non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown. Results In the present investigation, we verified that lncRNA-599554 had significantly higher expression at the anagen dermal papilla of cashmere goat SHF than that at telogen. Based on overexpression and knockdown techniques, we found that lncRNA-599554 contributes the inductive property of DPCs of cashmere goat, which was assessed by detecting the changes in the expression of several typical indictor genes in DPCs including ET-1, SCF, Versican, ALP, Lef1 and Ptc-1. Based on RNA pull-down assay, we verified that lncRNA-599554 directly interacted with chi-miR-15a-5p. Also, we showed that lncRNA-599554 positively regulated the Wnt3a expression in DPCs but which did not appear to involve its modulating of promoter methylation. Based on the use of Dual-luciferase reporter assays, our data indicated that lncRNA-599554 regulated the Wnt3a expression through chi-miR-15a-5p-mediated post-transcriptional level. Conclusions We showed that lncRNA-599554 contributes the inductive property of DPCs in cashmere goat which might be achieved through sponging chi-miR-15b-5p to promote the Wnt3a expression. The results from the present investigation provided a novel insight into the functional mechanism of lncRNA-599554 in the SHF regeneration of cashmere goat along with the formation and growth of cashmere fiber. How to cite: Yin RH, Wang YR, Zhao SJ, et al. LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.03.002

中文翻译：

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LncRNA-599554海绵miR-15a-5p通过正向调节绒山羊Wnt3a的表达促进真皮乳头细胞的诱导能力

摘要 背景 长链非编码 RNA (lncRNA) 作为转录后调节因子，被认为在绒山羊真皮乳头细胞 (DPC) 的诱导特性中起作用。此前，lncRNA-599554在羊绒山羊的次级毛囊（SHF）中被鉴定出来，但其功能意义尚不清楚。结果在本研究中，我们证实lncRNA-599554在绒山羊SHF生长期真皮乳头处的表达显着高于休止期。基于过表达和敲低技术，我们发现 lncRNA-599554 有助于绒山羊 DPC 的诱导特性，通过检测 DPC 中几个典型指示基因的表达变化进行评估，包括 ET-1、SCF、Versican、ALP 、Lef1 和 Ptc-1。基于 RNA 下拉分析，我们验证了 lncRNA-599554 直接与 chi-miR-15a-5p 相互作用。此外，我们发现 lncRNA-599554 正调控 DPC 中 Wnt3a 的表达，但似乎不涉及其对启动子甲基化的调节。基于双荧光素酶报告基因检测的使用，我们的数据表明 lncRNA-599554 通过 chi-miR-15a-5p 介导的转录后水平调节 Wnt3a 表达。结论 我们发现 lncRNA-599554 有助于绒山羊 DPC 的诱导特性，这可能通过海绵 chi-miR-15b-5p 促进 Wnt3a 表达来实现。本研究的结果为lncRNA-599554在羊绒山羊SHF再生以及羊绒纤维的形成和生长中的功能机制提供了新的见解。如何引用：尹 RH，Wang YR，Zhao SJ，等。LncRNA-599554 海绵 miR-15a-5p 通过正向调节绒山羊中 Wnt3a 的表达来促进真皮乳头细胞的诱导能力。电子 J 生物技术 2020；45。https://doi.org/10.1016/j.ejbt.2020.03.002