Abstract Background APETALA3 (AP3) has significant roles in petal and stamen development in accordance with the classical ABC model. Results The AP3 homolog, CDM19, from Chrysanthemum morifolium cv. Jinba was cloned and sequenced. Sequence and phylogenetic analyses revealed that CDM19 is of DEF/AP3 lineage possessing the characteristic MIKC-type II structure. Expression analysis showed that CDM19 was transcribed in petals and stamens of ray and disc florets with weak expression in the carpels. Ectopic expression of CDM19 in Arabidopsis wild-type background altered carpel development resulting in multi-carpel siliques. CDM19 could only partially rescue the Arabidopsis ap3‐–3 mutant. Conclusions Our results suggest that CDM19 may partially be involved in petal and stamen development in addition to having novel function in carpel development. How to cite: Githeng’u SK, Ding L, Zhao K, et al. Ectopic expression of Chrysanthemum CDM19 in Arabidopsis reveals a novel function in carpel development. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.03.001 .

中文翻译：

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菊花CDM19在拟南芥中的异位表达揭示了心皮发育的新功能

摘要 背景 APETALA3 (AP3) 根据经典的 ABC 模型在花瓣和雄蕊发育中具有重要作用。结果 来自 Chrysanthemum morifolium cv. 的 AP3 同源物 CDM19。金巴被克隆并测序。序列和系统发育分析表明，CDM19 属于 DEF/AP3 谱系，具有特征性 MIKC-II 型结构。表达分析表明，CDM19在射线和花盘小花的花瓣和雄蕊中转录，在心皮中表达较弱。CDM19 在拟南芥野生型背景中的异位表达改变了心皮发育，导致了多心皮长角果。CDM19 只能部分拯救拟南芥 ap3--3 突变体。结论我们的结果表明，除了在心皮发育中具有新功能外，CDM19 可能部分参与花瓣和雄蕊发育。如何引用：Githeng'u SK, Ding L, Zhao K, et al. 菊花 CDM19 在拟南芥中的异位表达揭示了心皮发育的新功能。电子 J 生物技术 2020；45。https://doi.org/10.1016/j.ejbt.2020.03.001。