Abstract Accurate diagnose of a disease in the early stage is critical to treat the disease properly. To this end, a multitude of biosensors with advanced technologies have been developed to detect the number of biomolecules precisely. In this work, we propose a method for extracting the Stern layer capacitance (Cstern) using the experimental data of silicon nanowire ion-sensitive field-effect transistors (ISFETs) to help improve the accurate detection of target molecules. The proposed method was applied to both pH and virus sensing scheme, and the Cstern value of pH and a virus were extracted as 32 and 26 μF/cm2, respectively. These findings indicated that the extracted Cstern was affected by the size of the ion and protein, which also was verified by a computer-aided simulation. These insights would be useful in the development of charge-based ISFET biosensors.

中文翻译：

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使用硅纳米线场效应晶体管在生物传感器检测中提取斯特恩层电容的实验

摘要 疾病的早期准确诊断是正确治疗疾病的关键。为此，已经开发了多种具有先进技术的生物传感器来精确检测生物分子的数量。在这项工作中，我们提出了一种利用硅纳米线离子敏感场效应晶体管 (ISFET) 的实验数据提取斯特恩层电容 (Cstern) 的方法，以帮助提高对目标分子的准确检测。所提出的方法适用于pH和病毒传感方案，pH和病毒的Cstern值分别为32和26 μF/cm2。这些发现表明提取的 Cstern 受离子和蛋白质大小的影响，这也得到了计算机辅助模拟的验证。