Purpose

To measure inflammatory mediators in the scleral lens fluid reservoir (FR) in healthy eyes and to compare them to basal tear samples after 8-hs (8h) and 4-days (4d) of scleral lens (SL) wear.

Methods

Fifteen normal, habitual soft contact lens wearers were fitted with 14.8- or 15.4-mm SLs (Zenlens, Alden Optical, USA). Basal ocular surface tears and FR samples were collected after 8h and 4d of daily SL wear. Levels of interleukin (IL) -4 and -8, matrix metalloproteinase (MMP)-7, -9, and -10, and tissue inhibitor of MMPs (TIMPs) 1–4 were measured in all samples using Luminex assays. Visual acuity, corneal and conjunctival staining, and comfort assessments were completed at the baseline, 8h and 4d time points.

Results

MMP-9 and MMP-10 were greater in FR than basal ocular surface tears. After 8h of SL wear, the median concentration of MMP-9 in the FR and basal tears were 62.7 and 15.2 ng/mL, respectively (p = 0.047). Likewise, MMP-10 was significantly greater in FR compared to basal tears, after 8h (25.8 ng/mL vs 2.8 ng/mL, p < 0.001) and 4d (2.1 ng/mL vs17.2 ng/mL, p = 0.047). IL-4 and IL-8 levels were greater in FR but not significantly at 8h (2.2 vs 3.1 ng/mL; and 0.1 vs 0.4 ng/mL, respectively) or 4d (0.9 vs 3.5 ng/mL; 0.0 vs 0.2 ng/mL). MMP-7 was not affected by SL wear after 8h (46.0 basal vs 54.4 ng/mL FR) or 4d (34.2 vs 87.5 ng/mL). Visual acuity, corneal and conjunctival staining did not change; comfort was reduced in SL compared to soft contact lens wear.

Conclusions

This is the first study to compare the FR with the basal ocular surface tears. MMP-9 and MMP-10 were elevated in the FR after several hours of SL wear, suggesting potential clinical implications of SL wear and deserves further investigation.

中文翻译：

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巩膜镜片磨损：测量储液器中的炎症

目的

测量健康眼睛巩膜液储库 (FR) 中的炎症介质，并将它们与巩膜镜 (SL) 佩戴 8 小时 (8 小时) 和 4 天 (4 天) 后的基础泪液样本进行比较。

方法

15 名正常、习惯性软性隐形眼镜佩戴者配戴 14.8 或 15.4 毫米 SL（Zenlens，Alden Optical，美国）。在每日 SL 佩戴 8 小时和 4 天后收集基础眼表泪液和 FR 样本。使用 Luminex 测定法测量了所有样品中白细胞介素 (IL) -4 和 -8、基质金属蛋白酶 (MMP)-7、-9 和 -10 以及 MMP 组织抑制剂 (TIMP) 1-4 的水平。在基线、8 小时和 4 天时间点完成视力、角膜和结膜染色以及舒适度评估。

结果

MMP-9 和 MMP-10 在 FR 中比基底眼表泪液更大。SL 磨损 8 小时后，FR 和基底泪液中 MMP-9 的中值浓度分别为 62.7 和 15.2 ng/mL (p = 0.047)。同样，在 8 小时（25.8 ng/mL 对 2.8 ng/mL，p < 0.001）和 4 天（2.1 ng/mL 对 17.2 ng/mL，p = 0.047）后，FR 中的 MMP-10 显着高于基础眼泪. IL-4 和 IL-8 水平在 FR 中更高，但在 8 小时（分别为 2.2 对 3.1 ng/mL；和 0.1 对 0.4 ng/mL）或 4 天（0.9 对 3.5 ng/mL；0.0 对 0.2 ng/mL）时不显着毫升）。MMP-7 在 8 小时（46.0 基础对 54.4 ng/mL FR）或 4 天（34.2 对 87.5 ng/mL）后不受 SL 磨损的影响。视力、角膜和结膜染色没有变化；与佩戴软性隐形眼镜相比，SL 的舒适度有所降低。

结论

这是第一项将 FR 与基底眼表泪液进行比较的研究。SL 磨损数小时后，FR 中 MMP-9 和 MMP-10 升高，表明 SL 磨损的潜在临床意义，值得进一步研究。