Purpose

During image acquisition, certain topographers require the addition of sodium fluorescein (NaFl) dye to the tear film. This study investigates the effect of NaFl dye on corneal topography and tear surface quality.

Method

The E300 corneal topographer (Medmont International Pty Ltd., Victoria, Australia) was used to measure ocular surface topography and quality of 57 eyes of 57 healthy individuals without dry eye symptoms, age 35.1 ± 15.2 years (mean ± standard deviation) ranging between 19 and 65 years. The mean of three simulated keratometry values, a variety of corneal shape descriptors, and Tear Film Surface Quality (TFSQ) were measured under three different conditions; without NaFl (baseline), with the addition of a single dose NaFl, and using a double dose of NaFl.

Results

Compared to baseline, the Inferior-Superior (IS) index decreased significantly after a single dose (P = 0.034) or double dose of NaFl (P = 0.030). The corneal surface was significantly more regular without NaFl (P = 0.003) or one insertion of NaFl (P = 0.024) when compared to two doses of NaFl. There was no association with age, or dry eye signs or symptoms on the variance observed in any of the indices between baseline, intervention I, and intervention II (P > 0.05). Agreement between corneal surface indices reduced following the addition of NaFl.

Conclusion

In comparison to measurements taken without an ocular dye, one dose of NaFl resulted in increased reliability and consistency in corneal topography measurements using the E300 topographer, but 2 doses decreased reliability and consistency. Practitioners ought to be aware that tear film surface regularity and inferior-superior corneal power changed significantly following the addition of NaFl in those with healthy corneas. Its effect in diseased corneas is unknown.

中文翻译：

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荧光素钠对眼前表面测量的影响。

目的

在图像采集过程中，某些地形图需要在泪膜中添加荧光素钠 (NaFl) 染料。本研究调查了 NaFl 染料对角膜地形和泪液表面质量的影响。

方法

使用E300角膜地形图仪（Medmont International Pty Ltd.，Victoria，Australia）测量57名无干眼症状的健康人的57只眼的眼表地形和质量，年龄35.1±15.2岁（平均值±标准差）19和 65 岁。在三种不同条件下测量了三个模拟角膜曲率值、各种角膜形状描述符和泪膜表面质量 (TFSQ) 的平均值；没有 NaFl（基线），添加单剂量 NaFl，并使用双剂量 NaFl。

结果

与基线相比，单次剂量 (P = 0.034) 或双剂量 NaFl (P = 0.030) 后，劣效 (IS) 指数显着下降。与两剂 NaFl 相比，没有 NaFl (P = 0.003) 或插入一次 NaFl (P = 0.024) 的角膜表面明显更规则。在基线、干预 I 和干预 II 之间的任何指标中观察到的方差与年龄或干眼体征或症状无关（P > 0.05）。添加 NaFl 后，角膜表面指数之间的一致性降低。

结论

与不使用眼部染料进行的测量相比，使用 E300 地形图仪进行角膜地形测量时，一剂 NaFl 提高了可靠性和一致性，但 2 剂降低了可靠性和一致性。从业者应该意识到，在角膜健康的人中，添加 NaFl 后，泪膜表面的规律性和下上层角膜屈光力发生了显着变化。其对患病角膜的影响尚不清楚。