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Antigen-labeled mesoporous silica-coated Au-core Pt-shell nanostructure: a novel nanoprobe for highly efficient virus diagnosis
Journal of Biological Engineering ( IF 5.7 ) Pub Date : 2019-11-14 , DOI: 10.1186/s13036-019-0220-1
Aiyun Li 1 , Lin Long 1, 2 , Fengshou Liu 1 , Jianbo Liu 1 , Xiaochun Wu 3 , Yinglu Ji 3
Affiliation  

As an emerging research area of artificial enzymes, nanozyme, the catalytic nanomaterials with enzyme-like characteristics, have attracted enormous attention in research. Here, a nanozyme probe has been realized by utilizing antigen-labeled mesoporous silica-encapsulated Au-core Pt-shell (Au@Pt@SiO2) nanostructures for the diagnosis of rubella virus (RV). Pt nanoparticles have been suggested to act as potent peroxidase mimetics with high activities. However, smaller Pt nanoparticles are very easily aggregated, which has negative effects on the catalytic activities. In this work, the use of gold nanorod as the support favours the well dispersion of the small Pt nanoparticles to improve the stability of them. Furthermore, the designed the silica shell could also isolate the recognition antigens from the surface reactive sites, retaining catalytic activity of the inner nanozyme. In addition, compared with antigen-labeled horseradish peroxidase (HRP), the antigen-labeled Au@Pt@SiO2 nanozyme was more stable and robust. A capture enzyme-linked immunosorbent assay (ELISA) for the determination of RV showed that the antigen-labeled Au@Pt@SiO2 nanozyme-based ELISA exhibited good sensitivity. The highly sensitive peroxidase-like activity of antigen-labeled Au@Pt@SiO2 nanozyme, along with their catalytic stability and robustness, can facilitate their utilization in biochemical assays and clinical diagnosis.

中文翻译:

抗原标记的介孔二氧化硅涂层金核铂壳纳米结构:一种用于高效病毒诊断的新型纳米探针

作为人工酶的新兴研究领域,纳米酶这种具有类酶特性的催化纳米材料,在研究中引起了极大的关注。在这里,利用抗原标记的介孔二氧化硅包裹的金核铂壳(Au@Pt@SiO2)纳米结构实现了用于诊断风疹病毒(RV)的纳米酶探针。Pt 纳米颗粒已被建议用作具有高活性的有效过氧化物酶模拟物。然而,较小的 Pt 纳米粒子很容易聚集,这对催化活性有负面影响。在这项工作中,使用金纳米棒作为载体有利于小 Pt 纳米粒子的良好分散,从而提高它们的稳定性。此外,设计的二氧化硅壳还可以将识别抗原与表面反应位点分离,保持内部纳米酶的催化活性。此外,与抗原标记的辣根过氧化物酶(HRP)相比,抗原标记的Au@Pt@SiO2纳米酶更加稳定和稳健。用于测定 RV 的捕获酶联免疫吸附试验 (ELISA) 表明,基于抗原标记的 Au@Pt@SiO2 纳米酶 ELISA 具有良好的灵敏度。抗原标记的 Au@Pt@SiO2 纳米酶的高度敏感的类过氧化物酶活性,以及​​它们的催化稳定性和鲁棒性,可以促进它们在生化检测和临床诊断中的应用。用于测定 RV 的捕获酶联免疫吸附试验 (ELISA) 表明,基于抗原标记的 Au@Pt@SiO2 纳米酶 ELISA 具有良好的灵敏度。抗原标记的 Au@Pt@SiO2 纳米酶的高度敏感的类过氧化物酶活性,以及​​它们的催化稳定性和鲁棒性,可以促进它们在生化检测和临床诊断中的应用。用于测定 RV 的捕获酶联免疫吸附试验 (ELISA) 表明,基于抗原标记的 Au@Pt@SiO2 纳米酶 ELISA 具有良好的灵敏度。抗原标记的 Au@Pt@SiO2 纳米酶的高度敏感的类过氧化物酶活性,以及​​它们的催化稳定性和鲁棒性,可以促进它们在生化检测和临床诊断中的应用。
更新日期:2020-04-22
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