HLA eplet matching is a novel approach to define acceptable HLA mismatches for transplant recipients. We performed an eplet analysis of three different transplant case‐series to determine if the available software programs gave accurate results. Eplet analysis was performed for three different transplant case‐series typed by NGS for all HLA class I and II loci. The three different HLA datasets were entered into both the HLAMatchmaker program (v2.1) and OLI Fusion MatchMaker (v4.2) software tools. Eplet results which were discordant were cross referenced against eplet registry and published HLA allele sequence data to determine the correct assignments. The comparison reveals that there was poor concordance between the two eplet programs. Analysis of the same donor/recipient pair often gave rise to different total eplet scores, incorrect eplet mismatches and antibody verification status, and both programs have eplets assigned to incorrect HLA alleles. Overall, the OLI Fusion MatchMaker eplet tool gave more accurate and useful eplet results. Eplet matching is still primarily a research tool. Before eplet matching can enter routine clinical practice further work is required to validate the accuracy of available eplet software programs. Incorrect eplet assignment could have serious adverse consequences in the clinical transplant setting.

中文翻译：

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不同的eplet软件程序会给出不一致和错误的结果：HLAMatchmaker与Fusion Matchmaker Eplet调用软件的分析。

HLA eplet匹配是为移植受体定义可接受的HLA错配的新颖方法。我们对三种不同的移植病例系列进行了eplet分析，以确定可用的软件程序是否给出了准确的结果。对所有HLA I类和II类位点的NGS分型的三个不同的移植病例系列进行了Eplet分析。将三个不同的HLA数据集输入到HLAMatchmaker程序（v2.1）和OLI Fusion MatchMaker（v4.2）软件工具中。将不一致的Eplet结果与eplet注册中心和已发布的HLA等位基因序列数据进行交叉引用，以确定正确的分配。比较表明，两个eplet程序之间的协调性很差。对同一个供体/受体对的分析通常会导致不同的总eplet分数，错误的eplet不匹配和抗体验证状态，并且两个程序都将eplet分配给错误的HLA等位基因。总体而言，OLI Fusion MatchMaker eplet工具提供了更加准确和有用的eplet结果。Eplet匹配仍然主要是一种研究工具。在eplet匹配可以进入常规临床实践之前，需要进行进一步的工作以验证可用eplet软件程序的准确性。错误的eplet分配可能在临床移植设置中产生严重的不良后果。在eplet匹配可以进入常规临床实践之前，需要进行进一步的工作以验证可用eplet软件程序的准确性。错误的eplet分配可能在临床移植设置中产生严重的不良后果。在eplet匹配进入常规临床实践之前，需要做进一步的工作来验证可用eplet软件程序的准确性。错误的eplet分配可能在临床移植设置中产生严重的不良后果。