RpoN1 and RpoN2 play different regulatory roles in virulence traits, flagellar biosynthesis, and basal metabolism in Xanthomonas campestris.,Molecular Plant Pathology

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RpoN1 and RpoN2 play different regulatory roles in virulence traits, flagellar biosynthesis, and basal metabolism in Xanthomonas campestris.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-04-13 , DOI: 10.1111/mpp.12938
Kaihuai Li ₁ , Guichun Wu ₂ , Yuling Liao ₃ , Quan Zeng ₄ , Haihong Wang ₃ , Fengquan Liu _{1,

2}

Affiliation

Homologous regulatory factors are widely present in bacteria, but whether homologous regulators synergistically or differentially regulate different biological functions remains mostly unknown. Here, we report that the homologous regulators RpoN1 and RpoN2 of the plant pathogen Xanthomonas campestris pv. campestris (Xcc) play different regulatory roles with respect to virulence traits, flagellar biosynthesis, and basal metabolism. RpoN2 directly regulated Xcc fliC and fliQ to modulate flagellar synthesis in X. campestris, thus affecting the swimming motility of X. campestris. Mutation of rpoN2 resulted in reduced production of biofilms and extracellular polysaccharides in Xcc. These defects may together cause reduced virulence of the rpoN2 mutant against the host plant. Moreover, we demonstrated that RpoN1 could regulate branched‐chain fatty acid production and modulate the synthesis of diffusible signal factor family quorum sensing signals. Although RpoN1 and RpoN2 are homologues, the regulatory roles and biological functions of these proteins were not interchangeable. Overall, our report provides new insights into the two different molecular roles that form the basis for the transcriptional specialization of RpoN homologues.

中文翻译：

RpoN1和RpoN2在油菜黄单胞菌的毒力特性，鞭毛生物合成和基础代谢中起不同的调节作用。

同源调控因子广泛存在于细菌中，但是同源调控因子是协同增效还是差异调控不同的生物学功能仍然未知。在这里，我们报告说植物病原体黄单胞菌PV同源调节剂RpoN1和RpoN2 。樟脑（Xcc）在毒力特性，鞭毛生物合成和基础代谢方面发挥不同的调节作用。RpoN2直接调节XCC的fliC和fliQ来调节鞭毛合成X.油菜，从而影响的游泳运动X.油菜。rpoN2突变导致Xcc中生物膜和细胞外多糖的产生减少。这些缺陷可能共同导致rpoN2突变体对宿主植物的毒性降低。此外，我们证明了RpoN1可以调节分支链脂肪酸的产生并调节可扩散信号因子家族群体感应信号的合成。尽管RpoN1和RpoN2是同源物，但这些蛋白质的调节作用和生物学功能不可互换。总体而言，我们的报告提供了对形成RpoN同源物转录专业化基础的两种不同分子作用的新见解。

更新日期：2020-04-13

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