The aim of this paper was to investigate the relevance of isoprostanoids i.e., F2-isoprostanes (F2-IsoPs), F4-neuroprostanes (F4-NeuroPs) and F2-dihomo-isoprostanes (F2-dihomo-IsoPs) in semen quality. Isoprostanoid levels were detected in semen of fertile and infertile men with varicocele or idiopathic infertility. Semen quality was assessed by light microscopy and transmission electron microscopy; the relationships between isoprostanes and semen parameters were also explored. F2-IsoPs levels were significantly different in the varicocele group compared to idiopathic infertile group and fertile men (P < 0.01 and P < 0.001 respectively). Moreover, F2-dihomo-IsoP values were significantly higher in varicocele group respect to fertile men (P < 0.05). No significant statistical differences were found regarding F4-NeuroP concentrations. In the whole population, F2-IsoPs positively correlated with F2-dihomo-IsoPs and both isoprostanoids showed a positive correlation with immaturity and a negative correlation with sperm motility. F2-IsoP levels were positively correlated with the percentage of immaturity in infertile varicocele groups (P < 0.01) whereas a significant relationship between F4-NeuroP values and the percentage of sperm necrosis was shown in idiopathic infertility group (P < 0.01). A significant negative correlation of F4-NeuroPs with sperm morphology was detected in infertile varicocele subjects (P < 0.05). This study suggests that isoprostanoid semen levels appear to be associated with male infertility being related to the sperm quality and confirming the important role of fatty acids profiling in human sperm maturation.

中文翻译：

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精浆中的F2-dihomo-IsoPs，F2-IsoPs和F4-NeuroPs与特发性不育症和精索静脉曲张的相关性。

本文的目的是研究异前列腺素类，即F2-异前列腺素（F2-IsoPs），F4-神经前列腺素（F4-NeuroPs）和F2-二高异异前列腺素（F2-dihomo-IsoPs）的相关性。在精索静脉曲张或特发性不育的可育和不育男性精液中检测到异前列腺素水平。精液质量通过光学显微镜和透射电子显微镜进行评估。还探讨了异前列腺素与精液参数之间的关系。与特发性不育组和可育男性相比，精索静脉曲张组中的F2-IsoPs水平显着不同（分别为P <0.01和P <0.001）。此外，精索静脉曲张组的F2-dihomo-IsoP值明显高于可育男性（P <0.05）。关于F4-NeuroP浓度没有发现显着的统计学差异。在整个人群中，F2-IsoPs与F2-dihomo-IsoPs正相关，并且两种异前列腺素类与未成熟度呈正相关，与精子活力呈负相关。不育性精索静脉曲张组中F2-IsoP水平与不成熟百分比呈正相关（P <0.01），而特发性不育组中F4-NeuroP值与精子坏死百分比之间呈显着相关性（P <0.01）。在不育精索静脉曲张受试者中检测到F4-NeuroPs与精子形态呈显着负相关（P <0.05）。这项研究表明，异前列腺素精液水平似乎与男性不育症有关，后者与精子质量有关，并证实了脂肪酸谱在人类精子成熟中的重要作用。F2-IsoPs与F2-dihomo-IsoPs正相关，并且两种异前列腺素类化合物均与未成熟度呈正相关，而与精子活力呈负相关。不育性精索静脉曲张组中F2-IsoP水平与不成熟百分比呈正相关（P <0.01），而特发性不育组中F4-NeuroP值与精子坏死百分比之间呈显着相关性（P <0.01）。在不育精索静脉曲张受试者中检测到F4-NeuroPs与精子形态呈显着负相关（P <0.05）。这项研究表明，异前列腺素精液水平似乎与男性不育症有关，后者与精子质量有关，并证实了脂肪酸谱在人类精子成熟中的重要作用。F2-IsoPs与F2-dihomo-IsoPs正相关，并且两种异前列腺素类化合物均与未成熟度呈正相关，与精子活力呈负相关。不育性精索静脉曲张组中F2-IsoP水平与不成熟百分比呈正相关（P <0.01），而特发性不育组中F4-NeuroP值与精子坏死百分比之间呈显着相关性（P <0.01）。在不育精索静脉曲张受试者中检测到F4-NeuroPs与精子形态呈显着负相关（P <0.05）。这项研究表明，异前列腺素精液水平似乎与男性不育症有关，后者与精子质量有关，并证实了脂肪酸谱在人类精子成熟中的重要作用。