Plasmid incompatibility is the inability of two plasmids to be stably maintained in one cell, resulting in loss of one of the plasmids in daughter cells. Dislodgement is a phenotypically distinct form of incompatibility, described as an imperfect reproduction, manifesting in rapid exclusion of a resident plasmid after superinfection. The relationship between plasmids of the phenotypic incompatibility groups IncB/O and IncZ is unclear. Their inability to co-exist was initially referred to as dislodgement while other research reached the conclusion that IncB/O and IncZ plasmids are incompatible. In this manuscript we re-evaluated the relationship between IncB/O and IncZ plasmids to settle these conflicting conclusions. We performed dislodgement testing of R16Δ (IncB/O) and pSFE-059 (IncZ) plasmids by electroporation in a bacterial cell and checked their stability. Stability tests of the obtained plasmid pair showed that the IncB/O plasmid was exclusively and almost completely lost from the heteroplasmid Escherichia coli population. Other IncB/O - IncZ pairs could not form a heteroplasmid population, using conjugation or electroporation. Our data supports the previous suggestion that IncB/O and IncZ plasmids may be considered phenotypically incompatible.

中文翻译：

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I型复杂质粒的不相容性和系统发育关系。

质粒不相容性是两种质粒不能在一个细胞中稳定维持，导致子细胞中一种质粒的丢失。去除是表型上不兼容的形式，被描述为不完美的复制，表现为在超级感染后快速排除了驻留质粒。表型不兼容组IncB / O和IncZ的质粒之间的关系尚不清楚。它们不能共存最初被称为清除，而其他研究得出的结论是IncB / O和IncZ质粒不相容。在本手稿中，我们重新评估了IncB / O和IncZ质粒之间的关系，以解决这些矛盾的结论。我们在细菌细胞中通过电穿孔进行了R16Δ（IncB / O）和pSFE-059（IncZ）质粒的置换测试，并检查了它们的稳定性。对获得的质粒对的稳定性测试表明，IncB / O质粒仅从异质大肠杆菌中完全消失。其他IncB / O-IncZ对无法通过结合或电穿孔形成异质体群体。我们的数据支持先前的建议，即IncB / O和IncZ质粒可能被视为表型不兼容。