Abstract Phytochemical investigation of Helleborus thibetanus led to the isolation of two new bufadienolides with a trans A/B ring fusion mode (1–2) and two known ones (3–4), three podophyllotoxins (5–7), two flavones (8 and 9), one phytoecdysteroid (10), one spirostane (11) and one pseudo-spirostane (12). The structures of the new compounds were identified using extensive spectroscopic analyses and the known compounds were identified by comparing the spectral data with those previously reported. Isolation of bufadienolides with a trans A/B ring fusion mode could be chemotaxonomic marker for the differentiation of H. thibetanus from the toad venom and other plants of this genus, on the other hand, co-occurrence of podophyllotoxin from H. thibetanus supported the inclusion of Berberidaceae and Ranunculaceae in the same monophyletic order Ranunculales based on the outgroup comparison and parsimony analysis.

中文翻译：

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玄参中蟾蜍二烯内酯和鬼臼毒素的共存

摘要 对藜芦的植物化学研究导致分离出两种具有反式 A/B 环融合模式的新蟾蜍二烯内酯 (1-2) 和两种已知的 (3-4)、三种鬼臼毒素 (5-7)、两种黄酮 (8和 9)、一种植物蜕皮激素 (10)、一种螺环烷 (11) 和一种假螺环烷 (12)。使用广泛的光谱分析鉴定了新化合物的结构，并通过将光谱数据与先前报道的数据进行比较来鉴定已知化合物。用反式 A/B 环融合模式分离蟾蜍二烯内酯可能是从蟾蜍毒液和该属的其他植物中分化黑蟾蜍的化学分类标记，另一方面，从蟾蜍毒中同时存在鬼臼毒素。