The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH‐adjusted and non‐pH‐adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro .

中文翻译：

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用乙酸根除鼓膜造口管上的生物膜：一项体外研究。

目的是通过体外实验评估乙酸对鼓膜造口管上生长的细菌生物膜的消灭作用。铜绿假单胞菌和金黄色葡萄球菌的生物膜在无菌鼓室造口管上生长24 h。将试管用各种浓度的乙酸溶液处理24小时。主要结局是治疗后细菌的生存力。使用共聚焦激光扫描显微镜检查在选定的鼓室造口管上是否存在始终附着的生物膜。将pH调节和非pH调节的培养基溶液均用作对照组。结果表明，用0.50％v / v乙酸完全清除了铜绿假单胞菌生物膜。金黄色葡萄球菌生物膜用1.25％v / v乙酸根除。仅低pH值会导致已经建立的生物膜生长减少，但不能消除。在conlusion，乙酸表现出对在无菌鼓膜造孔插管建立的生物膜根除的影响在体外。