It is indicated that malvidin has anti‐inflammatory and antioxidant effects on various cells, although the function of malvidin in preventing inflammatory reactions caused by lipopolysaccharide (LPS) in peripheral blood mononuclear cells (PBMCs) is still not known. The objective of this study was to examine the impact of malvidin on inflammatory responses and oxidative stress in PBMCs as caused by LPS. The present findings showed that LPS significantly increased the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA and protein release from PBMCs 22 hr after treatments. It was also revealed that increased levels in cytokine expression coincided with increased phosphorylation of JNK, P65‐NF‐κB, and IKKα/IKKβ. Also, the expression of IL‐6, TNF‐α, IL‐1β, and COX‐2 mRNA induced by LPS as well as secretion of protein in PBMC has been significantly decreased by pretreatment of malvidin. Importantly, pretreatment of the cells with malvidin completely abrogated the phosphorylation of P65‐NF‐κB, JNK, and IKKα/IKKβ in LPS treated cells. Malvidin protection against LPS‐induced inflammation was coupled with a decline in the levels of nitric oxide metabolite and malondialdehyde, along with an increase in the ferric reducing antioxidant power, total thiol activity, and also superoxide dismutase and glutathione peroxidase activity. In accordance with this finding, malvidin may represent a promising therapeutic agent for the prevention of inflammation in PBMCs.

中文翻译：

---

Malvidin 防止脂多糖诱导的人外周血单核细胞氧化应激和炎症

表明malvidin对多种细胞具有抗炎和抗氧化作用，但malvidin在预防外周血单核细胞（PBMCs）中脂多糖（LPS）引起的炎症反应中的作用尚不清楚。本研究的目的是检查马尔维丁对 LPS 引起的 PBMC 炎症反应和氧化应激的影响。目前的研究结果表明，LPS 显着增加 IL-6、TNF-α、IL-1β 和 COX-2 mRNA 的表达以及治疗后 22 小时 PBMC 的蛋白质释放。还发现细胞因子表达水平的增加与 JNK、P65-NF-κB 和 IKKα/IKKβ 的磷酸化增加一致。此外，IL-6、TNF-α、IL-1β、锦葵素的预处理显着降低了 LPS 诱导的 COX-2 mRNA 以及 PBMC 中蛋白质的分泌。重要的是，用锦葵素预处理细胞完全消除了 LPS 处理细胞中 P65-NF-κB、JNK 和 IKKα/IKKβ 的磷酸化。锦葵素对 LPS 诱导的炎症的保护与一氧化氮代谢物和丙二醛水平的下降相结合，同时铁还原抗氧化能力、总硫醇活性以及超氧化物歧化酶和谷胱甘肽过氧化物酶活性的增加。根据这一发现，malvidin 可能是预防 PBMC 炎症的一种有前途的治疗剂。和 IKKα/IKKβ 在 LPS 处理的细胞中。锦葵素对 LPS 诱导的炎症的保护与一氧化氮代谢物和丙二醛水平的下降相结合，同时铁还原抗氧化能力、总硫醇活性以及超氧化物歧化酶和谷胱甘肽过氧化物酶活性的增加。根据这一发现，malvidin 可能是预防 PBMC 炎症的一种有前途的治疗剂。和 IKKα/IKKβ 在 LPS 处理的细胞中。锦葵素对 LPS 诱导的炎症的保护与一氧化氮代谢物和丙二醛水平的下降相结合，同时铁还原抗氧化能力、总硫醇活性以及超氧化物歧化酶和谷胱甘肽过氧化物酶活性的增加。根据这一发现，malvidin 可能是预防 PBMC 炎症的一种有前途的治疗剂。