This study aimed to investigate the effect of long non‐coding RNA XLOC_003810 on the activation of CD4⁺ T cells and expression of PD‐1/PD‐L1 in patients with myasthenia gravis‐related thymoma (MG‐T). Thymus specimens and thymic mononuclear cells were obtained from MG and MG‐T patients or cardiac surgery patients undergoing thoracotomy who were selected as negative controls (NC). XLOC_003810 expression was examined using quantitative real‐time PCR (qRT‐PCR). Frequency of CD4⁺ T cells and proportion of CD4⁺PD‐1⁺ T cells and CD14⁺PD‐L1⁺ monocytes were quantified by flow cytometry. The release of inflammatory cytokines was measured by qRT‐PCR and enzyme‐linked immunosorbent assay. Compared with the NC group, expression of XLOC_003810, frequency of CD4⁺ T cells and the production of inflammatory cytokines were increased in patients with MG and MG‐T. XLOC_003810 overexpression significantly increased the frequency of CD4⁺ T cells, facilitated the production of inflammatory cytokines and decreased the proportion of CD4⁺PD‐1⁺ T cells and CD14⁺PD‐L1⁺ monocytes in the thymic mononuclear cells. In contrast, XLOC_003810 knockdown exerted the opposite effect. Together, XLOC_003810 promotes T cell activation and inhibits PD‐1/PD‐L1 pathway in patients with MG‐T.

中文翻译：

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LncRNA XLOC_003810促进重症肌无力相关胸腺瘤患者的T细胞活化并抑制PD-1 / PD-L1表达。

这项研究旨在探讨长非编码RNA XLOC_003810对重症肌无力相关胸腺瘤（MG-T）患者CD4 ⁺ T细胞活化和PD-1 / PD-L1表达的影响。胸腺标本和胸腺单核细胞是从MG和MG-T患者或接受开胸手术的心脏手术患者中选作阴性对照（NC）的。使用定量实时PCR（qRT-PCR）检查了XLOC_003810的表达。CD4 ⁺ T细胞的频率以及CD4 ⁺ PD-1 ⁺ T细胞和CD14 ⁺ PD-L1 ^+的比例单核细胞通过流式细胞仪定量。通过qRT-PCR和酶联免疫吸附测定法测定炎性细胞因子的释放。与NC组相比，MG和MG-T患者的XLOC_003810表达，CD4 ⁺ T细胞频率和炎性细胞因子的产生增加。XLOC_003810过表达显着增加了CD4 ⁺ T细胞的频率，促进了炎性细胞因子的产生，并降低了CD4 ⁺ PD-1 ⁺ T细胞和CD14 ⁺ PD-L1 ⁺的比例胸腺单核细胞中的单核细胞。相反，XLOC_003810的组合产生相反的效果。XLOC_003810共同促进MG-T患者的T细胞活化并抑制PD-1 / PD-L1途径。