Porcine deltacoronavirus infection alters bacterial communities in the colon and feces of neonatal piglets.,MicrobiologyOpen

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Porcine deltacoronavirus infection alters bacterial communities in the colon and feces of neonatal piglets.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-01 , DOI: 10.1002/mbo3.1036
Hai-Yan Li ₁ , Bing-Xiao Li ₁ , Qing-Qing Liang ₁ , Xiao-Hui Jin _{1,

2} , Lei Tang ₁ , Qing-Wen Ding _{1,

2} , Zhi-Xiang Wang ₁ , Zhan-Yong Wei _{1,

2}

Affiliation

Porcine deltacoronavirus (PDCoV) is a novel enteropathogenic coronavirus that causes watery diarrhea in piglets. Little is known regarding the alteration of the gut microbiota in PDCoV‐induced diarrhea piglets. In this study, 5‐day‐old piglets were experimentally infected with PDCoV strain CH‐01, and all piglets developed typical clinical disease, characterized by acute and severe watery diarrhea. Histologic lesions were limited to the villous epithelium of the duodenum and ileum. Gut microbiota profiles in the colon and feces of piglets inoculated with PDCoV were investigated using 16S rRNA sequencing. The results showed that PDCoV infection reduced bacterial diversity and significantly altered the composition of the microbiota from the phylum to the genus level in the colon and feces of piglets. Firmicutes (phylum), Lactobacillaceae (family), and Lactobacillus (genus) were significantly increased (p < .01), while the abundance of Bacteroidetes (phylum) was markedly reduced in the colon and feces of the PDCoV‐infected piglets (p < .01) when compared to those of the healthy piglets. Furthermore, microbial function prediction indicated that the changes in the intestinal flora also affected the nucleotide transport and metabolism, defense, translation, and transcription function of the intestinal microbiota. The current study provides new insight into the pathology and physiology of PDCoV.

中文翻译：

猪三角洲冠状病毒感染会改变新生仔猪结肠和粪便中的细菌群落。

猪三角洲冠状病毒（PDCoV）是一种新型的致肠病性冠状病毒，可导致仔猪出现水样腹泻。关于PDCoV引起的腹泻仔猪肠道微生物群改变的了解甚少。在这项研究中，对5天大的仔猪进行了实验性感染PDCoV株CH-01，所有仔猪均表现出典型的临床疾病，其特征是急性和严重的水样腹泻。组织学病变仅限于十二指肠和回肠的绒毛上皮。使用16S rRNA测序研究了接种PDCoV的仔猪结肠和粪便中的肠道菌群谱。结果表明，PDCoV感染减少了细菌的多样性，并显着改变了仔猪结肠和粪便中从门到属的微生物群组成。坚定（门），乳杆菌科（科）和（属）乳杆菌显着增加（p <.01），而被PDCoV感染的仔猪的结肠和粪便中的拟杆菌（门）的丰度明显降低（p <。 01）与健康仔猪相比。此外，微生物功能预测表明，肠道菌群的变化也影响了肠道菌群的核苷酸运输和代谢，防御，翻译和转录功能。当前的研究提供了对PDCoV的病理学和生理学的新见解。

更新日期：2020-04-01

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