AIMS Neuroinflammation can manifest upon infection with the neurotropic parasite Toxoplasma gondii (ME49), which can lead to brain injury and cognitive dysfunction. Rhoptry organelle proteins (ROPs) secreted by T gondii play critical roles in host invasion. METHODS AND RESULTS In this study, influenza virus-like particles (VLPs) expressing T gondii ROP4 or ROP13 were generated to assess vaccination-induced changes in intracranial pro-inflammatory cytokines and antibody responses upon T gondii challenge infection. Compared to ROP13 VLPs, ROP4VLPs vaccination significantly limited the production of pro-inflammatory cytokines IFN-γ and IL-6 in the brains of mice. Reduced pro-inflammatory cytokine responses by ROP4 VLPs and ROP13 VLPs correlated with significantly increased T gondii-specific IgG and IgA antibody responses in the brain, as well as IgG, IgG1 and IgM antibody responses in the sera. CONCLUSION We concluded that influenza T gondii VLP vaccination induces antibody responses in sera and brain, which may contribute to the significant reduction of neuroinflammation during T gondii infection.

中文翻译：

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弓形虫病毒样颗粒疫苗可减轻弓形虫感染后大脑中的炎症反应。

AIMS神经炎症可在感染了嗜神经性寄生虫弓形虫（ME49）后出现，可导致脑损伤和认知功能障碍。弓形虫分泌的Rhoptry细胞器蛋白（ROP）在宿主入侵中起关键作用。方法和结果在这项研究中，表达T型弓形虫ROP4或ROP13的流感病毒样颗粒（VLP）被产生，以评估疫苗诱导的T型弓形虫攻击感染后颅内促炎细胞因子的变化和抗体反应。与ROP13 VLP相比，ROP4VLP的疫苗接种显着限制了小鼠大脑中促炎性细胞因子IFN-γ和IL-6的产生。ROP4 VLP和ROP13 VLP降低的促炎细胞因子反应与脑中T弓形虫特异性IgG和IgA抗体反应以及IgG显着增加有关，血清中的IgG1和IgM抗体反应。结论我们得出结论，刚地弓形虫VLP疫苗接种可诱导血清和大脑中的抗体反应，这可能有助于在刚地弓形虫感染期间显着减少神经炎症。