Next‐generation sequencing (NGS) is the most modern sequencing technique that has revolutionized HLA typing, providing high‐resolution results with low ambiguity rates. This study aimed to show the experiences and challenges of an HLA laboratory in the validation process of the NGS methodology for HLA typing and show the use of this method for the study of HLA genetic diversity.

中文翻译：

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HLA的下一代测序：巴西人群中新的多态性的验证和鉴定。

下一代测序（NGS）是最现代的测序技术，它彻底改变了HLA分型，可提供高分辨率且低歧义率的结果。这项研究旨在展示HLA实验室在NGS方法进行HLA分型的验证过程中的经验和挑战，并展示该方法在HLA遗传多样性研究中的应用。