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Contemporary integrative taxonomy for sexually deprived protists: A case study of Trachelomonas (Euglenaceae) from western Ukraine
TAXON ( IF 3.0 ) Pub Date : 2020-04-10 , DOI: 10.1002/tax.12206
Anže Žerdoner Čalasan 1, 2 , Juliane Kretschmann 1 , Marc Gottschling 1
Affiliation  

As many other protist groups, euglenophytes are prone to false identification based solely on morphology because of a limited amount of morphological features and cryptic speciation. One of the supposedly completely asexual groups within the freshwater phototrophic representatives of euglenophytes is Trachelomonas, capable of forming an inorganic shell around its cell (i.e., the lorica). The International Code of Nomenclature for algae, fungi, and plants regulates the taxonomy not only of flowering plants, but explicitly also of phototrophic protists, and provides powerful tools to resolve various taxonomic challenges. To exemplify some of the problems and potential solutions, a number of Trachelomonas strains were collected from the muddy, lake‐rich region of Dobrostany and cultivated under stable laboratory conditions. Being a type locality of 58 unclarified Trachelomonas names, this region in western Ukraine is of great taxonomic importance. Based on light and electron microscopy, and on RAxML and MrBayes phylogenetics using multiple loci and a representative taxon sample, a detailed description of investigated strains and their systematic placement is provided. Morphologically, the strains differed slightly but consistently in minute characters such as size, lorica shape and ornamentation. The presently most comprehensive molecular tree of the Euglenaceae indicated to the existence of at least five different species present in the newly investigated samples, although they were collected from localities in very close vicinity to each other and at the same date. Based on morphological comparisons with type illustrations of species validly described 100 or more years ago, biological material was used to epitypify three names of Trachelomonas, eternally linking morphology with reliable genetic information. This taxonomic application is one of the powerful methods to clarify ambiguous scientific names, which has particular importance in character‐poor protists such as the euglenophytes.

中文翻译:

性剥夺者的当代综合分类法:以乌克兰西部的虎耳草科为例

与许多其他原生生物群体一样,由于数量有限的形态特征和隐性物种,裸藻植物倾向于仅基于形态学进行错误鉴定。一个euglenophytes的淡水光养代表内的假想完全无性基团的是Trachelomonas,能够形成围绕其的细胞(即,洛丽卡)无机外壳。藻类,真菌和植物的国际命名法不仅规范开花植物的分类法,而且明确规定了光养原生生物的分类法,并提供了解决各种分类学挑战的有力工具。为了举例说明一些问题和潜在的解决方案,许多气孔单胞菌从多布罗斯坦的泥泞,湖水丰富的地区收集了菌株,并在稳定的实验室条件下进行了栽培。是58个未阐明的气管单的类型局部性名称,乌克兰西部的这一地区具有重大的分类学意义。基于光学和电子显微镜,以及使用多个基因座和代表性分类单元样本的RAxML和MrBayes系统发育学,提供了对所研究菌株及其系统放置的详细描述。在形态上,菌株的大小,略有差异,但在微小特征上却始终如一。尽管最近一次采集的桉树科分子树是从彼此非常接近的地方并在同一日期采集的,但它们在新研究的样品中至少存在五个不同的物种。根据100年前或更有效地描述过的物种的形态学比较和类型说明,气孔单胞菌,将形态学与可靠的遗传信息永久地联系在一起。这种生物分类学的应用是澄清模棱两可的科学名称的有力方法之一,这对字符质量较差的原生生物(如裸藻类植物)尤为重要。
更新日期:2020-04-10
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