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Integrated omics analysis of sweat reveals an aberrant amino acid metabolism pathway in Vogt-Koyanagi-Harada disease.
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2020-04-14 , DOI: 10.1111/cei.13435 X Cui 1 , G Su 1 , L Zhang 1 , S Yi 1 , Q Cao 1 , C Zhou 1 , A Kijlstra 2 , P Yang 1
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2020-04-14 , DOI: 10.1111/cei.13435 X Cui 1 , G Su 1 , L Zhang 1 , S Yi 1 , Q Cao 1 , C Zhou 1 , A Kijlstra 2 , P Yang 1
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Vogt-Koyanagi-Harada (VKH) disease is an autoimmune disease leading to visual impairment. Its pathogenic mechanisms remain poorly understood. Our purpose was to investigate the distinctive protein and metabolic profiles of sweat in patients with VKH disease. In the present study, proteomics and metabolomics analysis was performed on 60 sweat samples (30 VKH patients and 30 normal controls) using liquid chromatography tandem mass spectrometry. Parallel reaction monitoring (PRM) analysis was used to validate the results of our omics analysis. In total, we were able to detect 716 proteins and 175 metabolites. Among them, 116 proteins (99 decreased and 17 increased) were observed to be significantly different in VKH patients when compared to controls. Twenty-one differentially expressed metabolites were identified in VKH patients, of which 18 included choline, L-tryptophan, betaine and L-serine were reduced, while the rest were increased. Our multi-omics strategy reveals an important role for the amino acid metabolic pathway in the pathogenesis of VKH disease. Significant differences in proteins and metabolites were identified in the sweat of VKH patients and, to some extent, an aberrant amino acid metabolism pathway may be a pathogenic factor in the pathogenesis of VKH disease.
中文翻译：
汗液的综合组学分析揭示了 Vogt-Koyanagi-Harada 病中异常的氨基酸代谢途径。
沃格特-小柳-原田 (VKH) 病是一种导致视力障碍的自身免疫性疾病。其致病机制仍知之甚少。我们的目的是研究 VKH 疾病患者汗液的独特蛋白质和代谢特征。在本研究中，使用液相色谱串联质谱法对 60 份汗液样本（30 名 VKH 患者和 30 名正常对照）进行了蛋白质组学和代谢组学分析。平行反应监测（PRM）分析用于验证我们的组学分析结果。总共，我们能够检测到 716 种蛋白质和 175 种代谢物。其中，与对照组相比，VKH 患者中有 116 种蛋白质（99 种减少，17 种增加）观察到显着差异。 VKH患者中鉴定出21种差异表达代谢物，其中18种包括胆碱、L-色氨酸、甜菜碱和L-丝氨酸减少，其余增加。我们的多组学策略揭示了氨基酸代谢途径在 VKH 疾病发病机制中的重要作用。 VKH患者汗液中蛋白质和代谢物存在显着差异，在某种程度上，异常的氨基酸代谢途径可能是VKH疾病发病机制的致病因素。
更新日期：2020-03-28
中文翻译：
汗液的综合组学分析揭示了 Vogt-Koyanagi-Harada 病中异常的氨基酸代谢途径。
沃格特-小柳-原田 (VKH) 病是一种导致视力障碍的自身免疫性疾病。其致病机制仍知之甚少。我们的目的是研究 VKH 疾病患者汗液的独特蛋白质和代谢特征。在本研究中，使用液相色谱串联质谱法对 60 份汗液样本（30 名 VKH 患者和 30 名正常对照）进行了蛋白质组学和代谢组学分析。平行反应监测（PRM）分析用于验证我们的组学分析结果。总共，我们能够检测到 716 种蛋白质和 175 种代谢物。其中，与对照组相比，VKH 患者中有 116 种蛋白质（99 种减少，17 种增加）观察到显着差异。 VKH患者中鉴定出21种差异表达代谢物，其中18种包括胆碱、L-色氨酸、甜菜碱和L-丝氨酸减少，其余增加。我们的多组学策略揭示了氨基酸代谢途径在 VKH 疾病发病机制中的重要作用。 VKH患者汗液中蛋白质和代谢物存在显着差异，在某种程度上，异常的氨基酸代谢途径可能是VKH疾病发病机制的致病因素。
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