Skin patch testing is still seen as the gold standard for the diagnosis of allergic hypersensitivity. For several metals and for patients with a suspected adverse reaction to their medical device implant material, patch testing can be unreliable. The current alternative to metal allergy patch testing is the in vitro lymphocyte proliferation test (LPT) using tritiated thymidine. This method is well‐established but requires handling of radioactive material, often uses heat‐inactivated allogenic human pooled serum and cannot determine T cell subsets.

中文翻译：

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非热灭活自体血清提高镍的体外 CFSE 淋巴细胞增殖试验 (LPT) 的准确性

皮肤斑贴试验仍被视为诊断过敏性超敏反应的金标准。对于几种金属以及对其医疗器械植入材料有疑似不良反应的患者，斑贴试验可能不可靠。目前金属过敏斑贴试验的替代方法是使用氚化胸苷的体外淋巴细胞增殖试验 (LPT)。这种方法已经成熟，但需要处理放射性物质，通常使用热灭活的同种异体人混合血清，并且无法确定 T 细胞亚群。