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Tryptophan targeted pulsed electric field treatment for enhanced immune activity in pine nut peptides.
Journal of Food Biochemistry ( IF 1.662 ) Pub Date : 2020-04-08 , DOI: 10.1111/jfbc.13224
Liangzi Sun,Shuyu Zhang,Huapeng Ju,Zhijie Bao,Songyi Lin

To investigate immune activity of pine nut peptides treated by PEF technology and mechanism of targeting immunoactive sitesits, immune regulatory active was evaluated by RAW 264.7 cells model and the structures of pine nut peptides were researched by fluorescence, CD, and 1D/2D NMR spectrum. These consequences showed the ability of macrophages to phagocytosis neutral red and the production of nitric oxide (NO) were improved after PEF treatment. KWFCT treated by PEF treatment with 40 kV/cm obtained the best immunocompetence. The CD spectroscopy showed that PEF could transform the secondary structures of pine nut peptides. The short‐range correlation between CγH (1.65 ppm) and CαH (3.35 ppm), and long‐range correlation between CαH (3.37 ppm) and NαH (8.07 ppm) were enhanced by PEF treatment. PEF treatment of tryptophan in the pine nut peptides enhanced the immunological activity of the pine nut peptides.
更新日期:2020-04-08

 

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