Tryptophan targeted pulsed electric field treatment for enhanced immune activity in pine nut peptides.,Journal of Food Biochemistry

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Tryptophan targeted pulsed electric field treatment for enhanced immune activity in pine nut peptides.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-04-08 , DOI: 10.1111/jfbc.13224
Liangzi Sun ₁ , Shuyu Zhang ₁ , Huapeng Ju ₁ , Zhijie Bao ₁ , Songyi Lin ₁

Affiliation

To investigate immune activity of pine nut peptides treated by PEF technology and mechanism of targeting immunoactive sitesits, immune regulatory active was evaluated by RAW 264.7 cells model and the structures of pine nut peptides were researched by fluorescence, CD, and 1D/2D NMR spectrum. These consequences showed the ability of macrophages to phagocytosis neutral red and the production of nitric oxide (NO) were improved after PEF treatment. KWFCT treated by PEF treatment with 40 kV/cm obtained the best immunocompetence. The CD spectroscopy showed that PEF could transform the secondary structures of pine nut peptides. The short‐range correlation between C_γH (1.65 ppm) and C_αH (3.35 ppm), and long‐range correlation between C_αH (3.37 ppm) and N_αH (8.07 ppm) were enhanced by PEF treatment. PEF treatment of tryptophan in the pine nut peptides enhanced the immunological activity of the pine nut peptides.

中文翻译：

色氨酸靶向脉冲电场治疗可增强松子肽的免疫活性。

为了研究PEF技术处理的松子肽的免疫活性和靶向免疫活性位点的机理，通过RAW 264.7细胞模型评估了免疫调节活性，并通过荧光，CD和1D / 2D NMR光谱研究了松子肽的结构。这些结果表明PEF处理后巨噬细胞吞噬中性红的能力和一氧化氮（NO）的产生得以改善。用40 kV / cm的PEF处理的KWFCT具有最佳的免疫能力。CD光谱表明PEF可以改变松子肽的二级结构。C之间的短距离相关_γ H（1.65 ppm的）和C _α H（3.35 ppm）设为长范围C之间的相关性_α（3.37 ppm的）H和N _αPEF处理可提高H（8.07 ppm）。PEF处理松子肽中色氨酸的方法增强了松子肽的免疫活性。

更新日期：2020-04-08

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