Effect of multi-frequency countercurrent ultrasound treatment on extraction optimization, functional and structural properties of protein isolates from Walnut (Juglans regia L.) meal.,Journal of Food Biochemistry

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Effect of multi-frequency countercurrent ultrasound treatment on extraction optimization, functional and structural properties of protein isolates from Walnut (Juglans regia L.) meal.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-31 , DOI: 10.1111/jfbc.13210
Moses Kwaku Golly _{1,

2} , Haile Ma _{1,

3} , Duan Yuqing ₁ , Liu Dandan ₁ , Janet Quaisie ₁ , Jamila Akter Tuli ₁ , Benjamin Kumah Mintah ₁ , Courage Sedem Dzah ₁ , Percival Delali Agordoh ₄

Affiliation

This study evaluated the effects of ultrasound treatment on walnut meal protein (WMP) extraction and techno‐functional properties. The Box‐Behnken Design (BBD) was adopted for the optimization of the traditional and ultrasound‐assisted extraction (UAE) processes. Standard protocols were used to assay the techno‐functional characteristics. The extraction models’ statistical results exhibited adequacy with the least desirability index of 95.8%. The UAE enhanced the WMP extraction yield, purity, and chemical score by 30.15%, 16.27%, and 9.74%, respectively, while reducing the extraction time by 25% over the control. The emulsion and foam stabilities and bulk density increased by 34.5%, 39.8%, and 6.1%, respectively, over the control. The α‐helix decreased while β‐sheet, β‐turns and random coil secondary structure components increased significantly (p < .05) by 95.76%, 101.3%, 105.1%, and 85.7% correspondingly. The dual‐frequency combination (20/40 kHz/kHz) was the best frequency mode. WMP could serve as a functional additive in manufactured foods as texture and flavor enhancer.

中文翻译：

多频逆流超声处理对核桃粕蛋白质分离物的提取优化，功能和结构特性的影响。

这项研究评估了超声处理对核桃粕蛋白（WMP）提取和技术功能特性的影响。采用Box-Behnken设计（BBD）来优化传统和超声辅助提取（UAE）工艺。使用标准协议分析技术功能特征。提取模型的统计结果显示出令人满意的结果，最低期望值指数为95.8％。阿联酋分别提高了WMP的提取率，纯度和化学评分30.15％，16.27％和9.74％，同时将提取时间比对照减少了25％。与对照相比，乳液和泡沫的稳定性和堆积密度分别提高了34.5％，39.8％和6.1％。α-螺旋下降，而β-折叠，β匝数和随机线圈二级结构组件分别显着增加（p <.05）95.76％，101.3％，105.1％和85.7％。双频组合（20/40 kHz / kHz）是最佳频率模式。WMP可以作为人造食品的功能添加剂，用作质地和风味增强剂。

更新日期：2020-03-31

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