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Integrated transcriptomic and proteomic analysis of the acetic acid stress in Issatchenkia orientalis.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-30 , DOI: 10.1111/jfbc.13203 Yingdi Li 1 , Zufang Wu 1, 2 , Ruoyun Li 1 , Yingjie Miao 1 , Peifang Weng 1 , Liping Wang 3
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-30 , DOI: 10.1111/jfbc.13203 Yingdi Li 1 , Zufang Wu 1, 2 , Ruoyun Li 1 , Yingjie Miao 1 , Peifang Weng 1 , Liping Wang 3
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Issatchenkia orientalis known as a multi‐tolerant non‐Saccharomyces yeast, which tolerant environmental stresses, exhibits potential in wine making and bioethanol production. It is essential for the growth of I. orientalis to tolerant acetic acid in the mixed cultures with Saccharomyces cerevisiae. In this work, RNA‐sequence and TMT (Tandem Mass Tag) were used to examine the comprehensive transcriptomic and proteomic profiles of I. orientalis in response to acetic acid. The results showed that 876 genes were identified differentially transcribed in I. orientalis genome and 399 proteins expressed in proteome after 4 hr acetic acid (90 mM, pH 4.5). The comprehensive analysis showed a series of determinants of acetic acid tolerance: Glycolysis and TCA cycle provide enough nicotinamide adenine dinucleotide to effectively convert acetic acid. Genes associated with potassium, iron, zinc, and glutathione synthesis were upregulated. The same changes of differentially expressed genes and proteins were mainly concentrated in chaperones, coenzyme, energy production, and transformation.
中文翻译：
整合的转录组学和蛋白质组学分析，侧柏中的乙酸。
Issatchenkia Orientalis被称为多耐受性非酿酒酵母，具有耐环境压力，在酿酒和生物乙醇生产中具有潜力。的是对于生长是必需的东方伊萨到宽容乙酸与混合培养酿酒酵母。在这项工作中，使用RNA序列和TMT（串联质谱标签）检查了东方伊士酵母对乙酸的综合转录组和蛋白质组学特征。结果表明，鉴定了876个基因在东方I.乙酸（90 mM，pH 4.5）4小时后在蛋白质组中表达基因组和399种蛋白质。全面的分析显示了一系列耐乙酸性的决定因素：糖酵解和TCA循环可提供足够的烟酰胺腺嘌呤二核苷酸来有效转化乙酸。与钾，铁，锌和谷胱甘肽合成相关的基因被上调。差异表达基因和蛋白质的相同变化主要集中在分子伴侣，辅酶，能量产生和转化上。
更新日期：2020-03-30
中文翻译：
整合的转录组学和蛋白质组学分析，侧柏中的乙酸。
Issatchenkia Orientalis被称为多耐受性非酿酒酵母，具有耐环境压力，在酿酒和生物乙醇生产中具有潜力。的是对于生长是必需的东方伊萨到宽容乙酸与混合培养酿酒酵母。在这项工作中，使用RNA序列和TMT（串联质谱标签）检查了东方伊士酵母对乙酸的综合转录组和蛋白质组学特征。结果表明，鉴定了876个基因在东方I.乙酸（90 mM，pH 4.5）4小时后在蛋白质组中表达基因组和399种蛋白质。全面的分析显示了一系列耐乙酸性的决定因素：糖酵解和TCA循环可提供足够的烟酰胺腺嘌呤二核苷酸来有效转化乙酸。与钾，铁，锌和谷胱甘肽合成相关的基因被上调。差异表达基因和蛋白质的相同变化主要集中在分子伴侣，辅酶，能量产生和转化上。
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