Antioxidant and anti-inflammatory activities of target anthocyanins di-glucosides isolated from Syzygium cumini pulp by high speed counter-current chromatography.,Journal of Food Biochemistry

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Antioxidant and anti-inflammatory activities of target anthocyanins di-glucosides isolated from Syzygium cumini pulp by high speed counter-current chromatography.
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-03-24 , DOI: 10.1111/jfbc.13209
Mohamed Abdin _{1,

2} , Yahya Saud Hamed _{1,

3} , Hafiz Muhammad Saleem Akhtar ₁ , Dan Chen ₁ , Guijie Chen ₁ , Peng Wan ₁ , Xiaoxiong Zeng ₁

Affiliation

High‐speed counter‐current chromatography (HSCCC) was utilized as an effective procedure for isolation of targeted three anthocyanins di‐glucosides from Syzygium cumini (SC) pulp by using an optimized biphasic successful combination of different solvents. The resulted fractions described by HPLC/ESI‐MS to be delphinidin 3,5‐diglucoside (DDG), petunidin 3,5‐diglucoside (PDG), and malvidin 3,5‐diglucoside (MDG). A weight of 150 mg of sample yielded 7.53, 22.68, and 39.09 mg for DDG, PDG, and MDG, respectively. It was stated that the target three anthocyanins possessed strong antioxidant activities. Furthermore, MDG exhibited definite advantages for inhibition of nitric oxide release and pro‐inflammatory mediators like mouse interleukin 6 (IL‐6), mouse interleukin (IL‐1β) and mouse tumor necrosis factor (TNF‐α) in lipopolysaccharide (LPS)‐induced RAW264.7 macrophages. The results propose that HSCCC can be utilized to separate highly antioxidative and anti‐inflammatory natural components from SC pulp.

中文翻译：

高速逆流色谱法分离自孜孜林果肉中目标花色苷二葡萄糖苷的抗氧化和抗炎活性。

高速逆流色谱法（HSCCC）被用作一种有效的方法，用于从孜然糖中分离出靶向的三种花色苷二葡糖苷（SC）纸浆通过使用不同溶剂的优化双相成功组合而获得。HPLC / ESI-MS所描述的馏分为翠菊素3,5-二葡萄糖苷（DDG），矮牵牛苷3,5-二葡萄糖苷（PDG）和麦维京3,5-二葡萄糖苷（MDG）。重量为150 mg的样品对于DDG，PDG和MDG分别产生7.53、22.68和39.09 mg。据指出，目标三种花色苷具有很强的抗氧化活性。此外，MDG在抑制一氧化氮释放和脂多糖（LPS）-中的小鼠白介素6（IL-6），小鼠白介素（IL-1β）和小鼠肿瘤坏死因子（TNF-α）等促炎性介质方面具有明显优势。诱导的RAW264.7巨噬细胞。结果表明，HSCCC可用于从SC纸浆中分离出高度抗氧化和抗炎的天然成分。

更新日期：2020-03-24

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