The aim of this study was to evaluate a colorimetric method, MIRONAUT-AM, for determining susceptibility testing of anidulafungin, amphotericin, voriconazole, and itraconazole by comparing the minimum inhibitory (effective) concentrations (MICs/MECs) obtained by this method to those generated by the reference Clinical Laboratory Standard Institute (CLSI) broth microdilution method. In sum, 78 clinical isolates of Aspergillus species, nine of them non-wild type (non-WT) with itraconazole MIC ranging from 2 mg/l to >16 mg/l, were tested against above antifungals. A. fumigatus ATCC 204305 was used as a reference strain, and test was performed in accordance with slightly modified yeast susceptibility testing instruction of the manufacture; conidia suspension inoculum and alamarBlue concentration were optimized. These same isolates were referred to Bristol Mycology reference laboratory and tested by CLSI method. The MICs and MECs generated by the two methods were compared using concordance analysis. MIRONAUT-AM showed significant concordance (P < .0001) with CLSI method, and overall agreement was high (≥90%). In addition, MIRONAUT-AM produced echinocandin MECs results within 18–24 hours incubation time and correctly detected all non-WT isolates except one isolate. This colorimetric method is very promising and appears to be a suitable alternative susceptibility testing method to labor intensive broth microdilution reference method for Aspergillus species.

中文翻译：

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MIRONAUT-AM 和 CLSI 肉汤微量稀释法对曲霉对四种常用抗真菌剂的抗真菌敏感性测试的比较评价

本研究的目的是评估比色法 MIRONAUT-AM，通过比较通过该方法获得的最小抑制（有效）浓度 (MICs/MECs) 与产生的浓度，确定阿尼芬净、两性霉素、伏立康唑和伊曲康唑的敏感性测试参考临床实验室标准协会（CLSI）肉汤微量稀释法。总之，对 78种曲霉属的临床分离株进行了针对上述抗真菌药物的测试，其中 9种是伊曲康唑 MIC 范围为 2 mg/l 至 >16 mg/l 的非野生型（非野生型）。烟曲霉以ATCC 204305为参比菌株，按照厂家稍作修改的酵母药敏试验说明书进行试验；优化了分生孢子悬浮接种物和alamarBlue浓度。这些相同的分离物被转至布里斯托尔真菌学参考实验室并通过 CLSI 方法进行测试。使用一致性分析比较两种方法产生的 MIC 和 MEC。MIRONAUT-AM 显示出显着的一致性 ( P < .0001) 与 CLSI 方法，总体一致性很高 (≥90%)。此外，MIRONAUT-AM 在 18-24 小时孵育时间内产生棘白菌素 MEC 结果，并正确检测除一种分离物外的所有非 WT 分离物。这种比色法非常有前景，似乎是替代劳动密集型肉汤微量稀释曲霉属参考方法的敏感性测试方法。