Coccidioidomycosis is a common cause of community-acquired pneumonia in endemic areas of the southwestern United States. Clinical presentations range from self-limited disease to severe, disseminated disease. As such, early and accurate diagnosis is essential to ensure appropriate treatment and monitoring. Currently available diagnostic testing has variable accuracy, particularly in certain patient populations, and new tests may offer improved accuracy for the diagnosis of coccidioidomycosis. Serum samples from patients with coccidioidomycosis and controls were tested for immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies using the MVista Coccidioides antibody detection EIA and two commonly used commercial enzyme immunoassay (EIA) kits: the IMMY Omega EIA and the Meridian Premier EIA. The sensitivity of the IgG antibody detection was 87.4% using the MVista test compared to 46.6% for IMMY and 70.9% for Meridian. The sensitivity for IgM antibody detection was 61.2% for the MVista test, 22.3% for IMMY and 29.1% for Meridian. For IgG antibody detection, specificity was 90% for the MVista EIA, 94.6% for IMMY, 96.4% for Meridian. For IgM antibody detection, specificity was 95.3% for the MVista test 98.2% for IMMY and 99.1% for Meridian. The MVista Coccidioides antibody EIA offers improved sensitivity, including among high-risk patient populations, for the detection of IgG and IgM antibodies in comparison to other currently available EIAs.

中文翻译：

---

三种抗球虫抗体酶免疫试剂盒对球虫病诊断的比较。

球孢子菌病是美国西南部流行地区社区获得性肺炎的常见原因。临床表现从自限性疾病到严重的弥漫性疾病。因此，早期和准确的诊断对于确保适当的治疗和监测至关重要。当前可用的诊断测试具有可变的准确性，尤其是在某些患者人群中，新的测试可能为球虫病的诊断提供更高的准确性。使用MVista球虫球菌检测了球虫病和对照患者的血清样品中的免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗体抗体检测EIA和两种常用的商业酶免疫分析（EIA）试剂盒：IMMY Omega EIA和Meridian Premier EIA。使用MVista测试，IgG抗体检测的灵敏度为87.4％，而IMMY和Meridian的灵敏度分别为46.6％和70.9％。对于MVista测试，IgM抗体检测的灵敏度为61.2％，对于IMMY为22.3％，对于子午线为29.1％。对于IgG抗体检测，MVista EIA的特异性为90％，IMMY的特异性为94.6％，子午线的特异性为96.4％。对于IgM抗体检测，MVista测试的特异性为95.3％，IMMY为98.2％，子午线为99.1％。与其他目前可用的EIA相比，MVista球虫类抗体EIA（包括高危患者人群）对IgG和IgM抗体的检测灵敏度更高。