ABSTRACT

Investigations into the potential for pharmacological inhibition of the aging process and the onset of age-related disease are increasingly garnering attention. Here, we analyzed the antiaging properties of natural compounds derived from several marine bacteria in vitro and in vivo using the fission yeast Schizosaccharomyces pombe. The Pseudoalteromonas flavipulchra STILL-33 extract exhibited high antioxidant and antiglycation activities in vitro. We then characterized two antioxidant active fractions isolated from this extract. In addition, we showed that the P. flavipulchra STILL-33 extract or either of its two active fractions (Fractions 1 and 2) could extend the longevity of fission yeast. Moreover, the particular extract and two active fractions were found to induce mitochondrial activity and to delay the G1 phase of the fission yeast cell cycle, perhaps by improving the aging process. The P. flavipulchra STILL-33 extract and Fraction 1 also increased the expression of the catalase-encoding ctt1+ gene and thereby decreased the reactive oxygen species level. Structural analysis showed that Fraction 1 was dominated by l-arginine and ipriflavone, and we showed indeed that the two corresponding commercial products increase the fission yeast lifespan. As for Fraction 2 was identified as the putative structure of butamben. Together, these results should facilitate the discovery of additional antiaging compounds from P. flavipulchra and ultimately the development of novel antiaging compounds for pharmaceutical use.

中文翻译：

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从海洋细菌黄假单胞菌STILL-33中分离的天然提取物及其馏分对粟酒裂殖酵母具有抗氧化和抗衰老作用

摘要

关于药理作用抑制衰老过程和与年龄有关的疾病发作的潜力的研究日益受到关注。在这里，我们使用裂殖酵母粟酒裂殖酵母分析了来自几种海洋细菌的天然化合物的体外和体内抗衰老特性。的假交替单胞flavipulchra STILL-33提取物显示出高的抗氧化和抗糖化活性的体外。然后，我们表征了从该提取物中分离出的两个抗氧化剂活性成分。此外，我们证明了黄萎病菌STILL-33提取物或其两个活性级分之一（组分1和2）可以延长裂殖酵母的寿命。此外，发现特定的提取物和两个活性级分可以诱导线粒体活性，并可能通过改善衰老过程来延迟裂变酵母细胞周期的G1期。的P. flavipulchra STILL-33提取物和级分1还增加了编码过氧化氢酶的表达CTT1 +基因，并由此降低了活性氧种的水平。结构分析表明，分数1由l控制。-精氨酸和依普黄酮，我们的确证明了两种相应的商业产品可延长裂变酵母的寿命。至于级分2，被确定为丁苯本的推定结构。总之，这些结果将有助于从黄果体育中发现其他抗衰老化合物，并最终开发用于药物用途的新型抗衰老化合物。