Layered and multi-input autonomous dynamic control strategies for metabolic engineering.,Current Opinion in Biotechnology

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Layered and multi-input autonomous dynamic control strategies for metabolic engineering.
Current Opinion in Biotechnology ( IF 7.1 ) Pub Date : 2020-04-12 , DOI: 10.1016/j.copbio.2020.02.015
Christina V Dinh ₁ , Kristala Lj Prather ₁

Affiliation

Metabolic engineering seeks to reprogram cells to efficiently produce value-added chemicals. Traditionally, this is achieved by overexpressing the production pathway and/or knocking out competing endogenous pathways. However, limitations in some pathways are more effectively addressed through dynamic metabolic flux control to favor different cellular objectives over the course of the fermentation. Dynamic control circuits can autonomously actuate changes in metabolic fluxes in response to changing fermentation conditions, cell density, or metabolite concentrations. In this review, we discuss recent studies focused on multiplexed autonomous strategies which (1) combine regulatory circuits to control metabolic flux at multiple nodes or (2) respond to more than one input signal. These strategies have the potential to address challenging pathway scenarios, actuate more complex response profiles, and improve the specificity of the criteria that actuate the dynamic response.

中文翻译：

代谢工程的分层和多输入自主动态控制策略。

代谢工程学试图对细胞进行重新编程，以有效地生产增值化学品。传统上，这是通过过度表达生产途径和/或敲除竞争性内源途径来实现的。然而，通过动态代谢通量控制以在发酵过程中有利于不同的细胞目标，可以更有效地解决某些途径的局限性。动态控制电路可以响应不断变化的发酵条件，细胞密度或代谢物浓度，自动启动代谢通量的变化。在这篇综述中，我们讨论了针对多重自主策略的最新研究，这些策略是（1）组合调节电路以控制多个节点的代谢通量，或者（2）响应多个输入信号。

更新日期：2020-04-20

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