A key to effective insect pest management and insecticide resistance management is to provide growers with a range of new tools as potential alternatives to existing compounds or approaches. Sulfoxaflor (Isoclast™ active) is a new sulfoximine insecticide which is active on a broad range of sap-feeding insects, including species that have reduced susceptibility to currently used insecticides, such as imidacloprid from the neonicotinoid class. Sulfoxaflor (SFX) and imidacloprid (IMI) were tested in laboratory bioassays to compare the susceptibility of field populations of green peach aphid, Myzus persicae (Sulzer), exhibiting varying degrees of resistance involving an alteration (R81T) to the insect nicotinic acetylcholine receptor. The LC50 values for M. persicae exposed to SFX ranged from 0.09 to 1.31 (mg litre-1), whereas when the same populations were exposed to IMI the LC50 values ranged from 0.6 to 76.2 (mg litre-1). M. persicae were significantly more sensitive to SFX as compared to IMI for nine of the 13 populations tested. For M. persicae populations confirmed to be homozygous susceptible (ss) or heterozygous rs) for the R81T point mutation, there was no significant differences in the observed LC50 values for either SFX or IMI relative to the susceptible reference population (15LP1). However, in all M persicae populations that were homozygous (rr) for the R81T point mutation, susceptibility was significantly less to IMI as compared to the reference population with resistance ratios ranging from 22.1 to 63.5-fold. In contrast, only one homozygous resistant population (15MP9) exhibited a statistically significant change in susceptibility (RR = 10-fold) to SFX as compared to the reference population, which was far less than the 56-fold observed for imidacloprid in that same population. Thus, this study indicates there is no specific correlation between the laboratory efficacy of SFX and IMI in field collected populations in Spain displaying varying degrees of resistance to IMI. Furthermore, the presence of target site resistance in M. persicae to IMI, in the form of the R81T mutation, does not a priori translate to a reduction in sensitivity to sulfoxaflor. Consequently, SFX can be an effective tool for use in insect pest management programs for green peach aphid. These data also serve as a baseline reference for green peach aphid sensitivity to SFX prior to commercial uses in Spain.

中文翻译：

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氟啶虫胺在实验室中对抗吡虫啉抗性和易感群体桃蚜的功效，桃蚜：R81T 突变对烟碱型乙酰胆碱受体的影响

有效害虫管理和杀虫剂抗性管理的关键是为种植者提供一系列新工具，作为现有化合物或方法的潜在替代品。Sulfoxaflor（Isoclast™ 活性成分）是一种新型亚砜亚胺杀虫剂，对多种以树液为食的昆虫具有活性，包括对当前使用的杀虫剂（如新烟碱类吡虫啉）敏感性降低的物种。在实验室生物测定中测试了氟啶虫胺 (SFX) 和吡虫啉 (IMI)，以比较田间桃蚜、桃蚜 (Sulzer) 种群的易感性，它们表现出不同程度的抗性，包括对昆虫烟碱型乙酰胆碱受体的改变 (R81T)。M. persicae 暴露于 SFX 的 LC50 值范围从 0.09 到 1.31 (mg litre-1)，而当相同的人群暴露于 IMI 时，LC50 值范围从 0.6 到 76.2（mg litre-1）。M. persicae 与 IMI 相比，对于测试的 13 个种群中的 9 个，对 SFX 的敏感性明显更高。对于 R81T 点突变确认为纯合易感 (ss) 或杂合 rs) 的桃蚜种群，相对于易感参考种群 (15LP1)，SFX 或 IMI 的观察到的 LC50 值没有显着差异。然而，在 R81T 点突变纯合 (rr) 的所有桃蚜种群中，与参考种群相比，对 IMI 的易感性显着降低，耐药率范围为 22.1 至 63.5 倍。相比之下，与参考群体相比，只有一个纯合抗性群体 (15MP9) 对 SFX 的易感性表现出统计学上的显着变化（RR = 10 倍），远低于在同一群体中观察到的吡虫啉的 56 倍。因此，这项研究表明，在西班牙现场采集的人群中，SFX 和 IMI 的实验室功效之间没有特定的相关性，显示出不同程度的 IMI 抗性。此外，桃核对 IMI 的靶位点抗性的存在，以 R81T 突变的形式，并不先验地转化为对氟啶虫胺的敏感性降低。因此，SFX 可以成为用于桃蚜虫害管理计划的有效工具。