MicroRNAs (miRNAs), a type of post-transcriptional regulators, exert a crucial role in the malignant progression of laryngeal squamous cell carcinoma (LSCC). MicroRNA-330-3p (miR-330-3p), a recently identified tumor-associated miRNA, is implicated in multiple cancers. Yet, the relevance of miR-330-3p in LSCC remains unexplored. The findings of our study demonstrated a lower expression of miR-330-3p in LSCC. Functional assays revealed that upregulation of miR-330-3p marked restricted the proliferation, colony formation and invasion of LSCC cells. Transformer-2 protein homolog beta (Tra2β) was identified as a target gene of miR-330-3p. An inverse correlation between miR-330-3p and Tra2β mRNA expression was evidenced in LSCC specimens. The upregulation of miR-330-3p significantly repressed Tra2β expression and the phosphorylation of the Akt protein. In addition, Tra2β overexpression markedly abrogated the tumor suppressive role of miR-330-3p in LSCC cells. Overall, our results uncovered that miR-330-3p exerted a tumor-inhibition function in LSCC through targeting Tra2β to inhibit Akt activation.

中文翻译：

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MicroRNA-330-3p通过下调Tra2β介导的Akt信号传导抑制喉鳞状细胞癌的增殖和侵袭。

MicroRNA（miRNA）是转录后调节剂的一种，在喉鳞状细胞癌（LSCC）的恶性进展中起关键作用。MicroRNA-330-3p（miR-330-3p）是最近发现的与肿瘤相关的miRNA，与多种癌症有关。但是，miR-330-3p在LSCC中的相关性尚未得到探索。我们研究的结果表明，miR-330-3p在LSCC中的表达较低。功能测定表明，miR-330-3p的上调标志着LSCC细胞的增殖，集落形成和侵袭受到限制。Transformer-2蛋白同源物beta（Tra2β）被确定为miR-330-3p的靶基因。在LSCC标本中证实了miR-330-3p与Tra2βmRNA表达呈负相关。miR-330-3p的上调显着抑制了Tra2β的表达和Akt蛋白的磷酸化。此外，Tra2β的过表达显着消除了miR-330-3p在LSCC细胞中的肿瘤抑制作用。总体而言，我们的结果揭示了miR-330-3p通过靶向Tra2β抑制Akt激活而在LSCC中发挥了肿瘤抑制功能。