In this study, we synthesized for the first time a bimetal-based inorganic-carbonic anhydrase (CA) hybrid nanoflower to immobilize CA using Cu²⁺ and Zn²⁺ instead of single metal ion. Subsequently, the synthesized bimetallic hybrid nanoflowers (CANF) were embedded into the poly(vinyl alcohol) (PVA)-chitosan (CS) hydrogel networks to obtain PVA/CS@CANF hydrogel membrane. The CANF exhibited a significantly higher activity recovery of 70 % compared with 35 % with CA/Zn₃(PO₄)₂ hybrid nanoflowers and 10 % with CA/Cu₃(PO₄)₂ hybrid nanoflowers. The PVA/CS@CANF hydrogel membrane possessed excellent mechanical strength, high catalytic activity, and were easy to flow out without centrifugation or filtration. At the same time, the PVA/CS@CANF displayed higher thermostability, storage stability, and pH stability than free CA and CANF, and superior reusability and CO₂ capture capacity. The hydrogel membrane maintained more than 75 % of its original activity after 8 cycles. However, CANF only maintained 12 % of its original activity. Furthermore, the amount of CaCO₃ produced by PVA/CS@CANF membrane was 9.0-fold and 2.0-fold compared with free CA and CANF, respectively. Therefore, This approach to synthesizing bimetallic-based protein hybrid hydrogel membrane could have a bright future in CO₂ capture.

在这项研究中，我们首次合成了基于双金属的无机碳酸酐酶（CA）杂化纳米花，使用Cu ²⁺和Zn ²⁺代替单金属离子来固定CA。随后，将合成的双金属杂化纳米花（CANF）嵌入聚乙烯醇（PVA）-壳聚糖（CS）水凝胶网络中，以获得PVA / CS @ CANF水凝胶膜。所述CANF表现出70％的显著更高的活性恢复与35％相比CA /锌₃（PO ₄）_2个的混合纳米花，并用CA / Cu的10％₃（PO ₄）₂杂种纳米花。PVA / CS @ CANF水凝胶膜具有优异的机械强度，高催化活性，并且不经离心或过滤便易于流出。同时，与游离的CA和CANF相比，PVA / CS @ CANF具有更高的热稳定性，储存稳定性和pH稳定性，并具有出色的可重复使用性和CO ₂捕获能力。在8个循环后，水凝胶膜保持其原始活性的75％以上。但是，CANF仅保留了其原始活动的12％。此外，与游离CA和CANF相比，PVA / CS @ CANF膜产生的CaCO ₃量分别为9.0倍和2.0倍。因此，这种合成基于双金属的蛋白质杂化水凝胶膜的方法在CO ₂方面具有广阔的前景 捕获。