Pulsed electromagnetic field (PEMF) and whole body vibration (WBV) interventions are expected to be important strategies for management of osteoarthritis (OA). The aim of the study was to investigate the comparative effectiveness of PEMF versus WBV on cartilage and subchondral trabecular bone in mice with knee OA (KOA) induced by surgical destabilization of the medial meniscus (DMM). Forty 12-week-old male C57/BL mice were randomly divided into four groups (n = 10): Control, OA, PEMF, and WBV. OA was induced (OA, PEMF, and WBV groups) by surgical DMM of right knee joint. Mice in PEMF group received 1 h/day PEMF exposure with 75 Hz, 1.6 mT for 4 weeks, and the WBV group was exposed to WBV for 20 min/day with 5 Hz, 4 mm, 0.3 g peak acceleration for 4 weeks. Micro-computed tomography (micro-CT), histology, and immunohistochemistry analyses were performed to evaluate the changes in cartilage and microstructure of trabecular bone. The bone volume fraction (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) increased, and bone surface/bone volume (BS/BV) decreased by micro-CT analysis in PEMF and WBV groups. The Osteoarthritis Research Society International (OARSI) scores in PEMF and WBV groups were significantly lower than in the OA group. Immunohistochemical results showed that PEMF and WBV promoted expressions of Aggrecan, and inhibited expressions of IL-1β, ADAMTS4, and MMP13. Superior results are seen in PEMF group compared with WBV group. Both PEMF and WBV were effective, could delay cartilage degeneration and preserve subchondral trabecular bone microarchitecture, and PEMF was found to be superior to WBV. Bioelectromagnetics. 2020;41:298-307 © 2020 Bioelectromagnetics Society.

中文翻译：

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脉冲电磁场​​与全身振动在膝关节骨关节炎小鼠软骨和软骨下小梁骨上的关系。

脉冲电磁场​​（PEMF）和全身振动（WBV）干预有望成为治疗骨关节炎（OA）的重要策略。这项研究的目的是研究PEMF与WBV在内侧半月板（DMM）手术不稳定引起的膝OA（KOA）小鼠的软骨和软骨下小梁骨中的比较有效性。将40只12周大的雄性C57 / BL小鼠随机分为四组（n = 10）：对照组，OA，PEMF和WBV。右膝关节DMM诱发OA（OA，PEMF和WBV组）。PEMF组的小鼠接受1 h / day的PEMF暴露，频率为75 Hz，1.6 mT，持续4周，而WBV组则暴露于WBV，频率为5 Hz，4 mm，0.3 g，峰值加速度为20 min / day，持续4周。微型计算机断层扫描（micro-CT），组织学，并进行免疫组化分析以评估小梁骨软骨和微结构的变化。通过微型CT分析，PEMF和WBV组的骨体积分数（BV / TV），小梁厚度（Tb.Th）和小梁数目（Tb.N）增加，而骨表面/骨体积（BS / BV）减少。PEMF和WBV组的国际骨关节炎研究协会（OARSI）评分显着低于OA组。免疫组织化学结果显示PEMF和WBV促进Aggrecan的表达，并抑制IL-1β，ADAMTS4和MMP13的表达。PEMF组与WBV组相比效果更好。PEMF和WBV均有效，可延迟软骨变性并保留软骨下小梁骨微结构，并且PEMF优于WBV。生物电磁学。