Atg3-catalyzed transferring of Atg8 to phosphatidylethanolamine (PE) in the phagophore membrane is essential for autophagy. Previous studies have demonstrated that this process requires Atg3 to interact with the phagophore membrane via its N-terminal amphipathic helix. In this study, by using combined biochemical and biophysical approaches, our data showed that in addition to binding to the membranes, Atg3 attenuates lipid diffusion and enriches lipid molecules with smaller headgroup. Our data suggest that Atg3 promotes Atg8 lipidation via altering lipid diffusion and rearrangement.

中文翻译：

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Atg3通过改变脂质扩散和重排促进Atg8脂化。

Atg3催化的Atg8向吞噬细胞膜中的磷脂酰乙醇胺（PE）的转移对于自噬至关重要。先前的研究表明，此过程需要Atg3通过其N末端两亲性螺旋与吞噬膜相互作用。在这项研究中，通过结合使用生物化学和生物物理方法，我们的数据表明，Atg3除了与膜结合外，还可以减弱脂质扩散并以较小的头基富集脂质分子。我们的数据表明Atg3通过改变脂质的扩散和重排促进Atg8的脂化。