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MicroRNA-223-5p targets long non-coding RNA TP73 antisense RNA1 to promote the invasion of gastric cancer.
Human Cell ( IF 3.4 ) Pub Date : 2020-04-04 , DOI: 10.1007/s13577-020-00349-3
Junhui Liu 1 , Haijiang Wang 2 , Xinhua Liao 2
Affiliation  

Long non-coding RNA (lncRNA) TP73 antisense RNA 1 (TP73-AS1) has been characterized as an oncogenic lncRNA in GC. However, by analyzing The Cancer Genome Atlas (TCGA) dataset we observed the downregulation of TP73-AS1 in GC. In addition, TP73-AS1 is predicted to interact with microRNA-223-5p (miR-223-5p), which is also a critical player in cancer biology. This study was therefore carried out to investigate the roles of miR-223-5p and TP73-AS1 in gastric cancer (GC) and to explore the interactions between them. In this study, 68 GC patients were included as research subjects. Expression of miR-223-5p and TP73-AS1 was analyzed by RT-qPCR. Dual-luciferase assay and overexpression experiments were used to analyze gene interactions. Transwell assays were used to analyze cell invasion and migration. We found that miR-223-5p was upregulated and TP73-AS1 was downregulated in GC and they were inversely correlated. Altered miR-223-5p and TP73-AS1 expression predicted poor disease-specific survival. Dual-luciferase assay showed that miR-223-5p may bind TP73-AS1 and overexpression experiments showed that miR-223-5p overexpression downregulated TP73-AS1 in gastric cancer cells. Cell invasion and migration assays showed that miR-223-5p could promote the invasion and migration of gastric cancer cells, while TP73-AS1 could inhibit the invasion and migration of gastric cancer cells. In addition, miR-223-5p attenuated the effects of TP73-AS1 overexpression. Therefore, miR-223-5p may target TP73-AS1 to promote the invasion and migration of gastric cancer patients.

中文翻译:

MicroRNA-223-5p靶向长非编码RNA TP73反义RNA1,以促进胃癌的侵袭。

长非编码RNA(lncRNA)TP73反义RNA 1(TP73-AS1)已被鉴定为GC中的致癌lncRNA。但是,通过分析癌症基因组图谱(TCGA)数据集,我们观察到GC中TP73-AS1的下调。此外,预计TP73-AS1与microRNA-223-5p(miR-223-5p)相互作用,而microRNA-223-5p在癌症生物学中也是至关重要的角色。因此,本研究旨在研究miR-223-5p和TP73-AS1在胃癌(GC)中的作用,并探讨它们之间的相互作用。在这项研究中,有68名GC患者被纳入研究对象。通过RT-qPCR分析miR-223-5p和TP73-AS1的表达。使用双重荧光素酶测定和过表达实验来分析基因相互作用。Transwell分析用于分析细胞侵袭和迁移。我们发现,GC中miR-223-5p被上调,而TP73-AS1被下调,并且它们呈负相关。改变的miR-223-5p和TP73-AS1表达预测疾病特异性存活率低。双荧光素酶检测显示miR-223-5p可能与TP73-AS1结合,而过表达实验表明miR-223-5p的过表达下调了胃癌细胞中TP73-AS1的表达。细胞侵袭和迁移分析表明,miR-223-5p可以促进胃癌细胞的侵袭和迁移,而TP73-AS1可以抑制胃癌细胞的侵袭和迁移。此外,miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。改变的miR-223-5p和TP73-AS1表达预测疾病特异性存活率低。双荧光素酶检测显示miR-223-5p可能与TP73-AS1结合,而过表达实验表明miR-223-5p的过表达下调了胃癌细胞中TP73-AS1的表达。细胞侵袭和迁移分析表明,miR-223-5p可以促进胃癌细胞的侵袭和迁移,而TP73-AS1可以抑制胃癌细胞的侵袭和迁移。此外,miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。改变的miR-223-5p和TP73-AS1表达预测疾病特异性存活率低。双荧光素酶检测显示miR-223-5p可能与TP73-AS1结合,而过表达实验表明miR-223-5p的过表达下调了胃癌细胞中TP73-AS1的表达。细胞侵袭和迁移分析表明,miR-223-5p可以促进胃癌细胞的侵袭和迁移,而TP73-AS1可以抑制胃癌细胞的侵袭和迁移。此外,miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。双荧光素酶检测显示miR-223-5p可能与TP73-AS1结合,而过表达实验表明miR-223-5p的过表达下调了胃癌细胞中TP73-AS1的表达。细胞侵袭和迁移分析表明,miR-223-5p可以促进胃癌细胞的侵袭和迁移,而TP73-AS1可以抑制胃癌细胞的侵袭和迁移。此外,miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。双荧光素酶检测显示miR-223-5p可能与TP73-AS1结合,而过表达实验表明miR-223-5p的过表达下调了胃癌细胞中TP73-AS1的表达。细胞侵袭和迁移分析表明,miR-223-5p可以促进胃癌细胞的侵袭和迁移,而TP73-AS1可以抑制胃癌细胞的侵袭和迁移。此外,miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。miR-223-5p减弱了TP73-AS1过表达的作用。因此,miR-223-5p可能靶向TP73-AS1来促进胃癌患者的侵袭和迁移。
更新日期:2020-04-04
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