Gibberellins (GAs) mediated elongation growth and its associated proteomics in rice is relevant to crop productivity in many diversified ecological conditions. There is dearth of precise investigations conducted on proteome dynamics involved in leaf sheath elongation in both wild and mutant rice plants in response to GAs treatments. To explore the molecular mechanism of GA-induced leaf sheath elongation in rice, we conducted a comparative proteomics analysis of wild type rice and a GA biosynthesis deficient mutant osko2 . The mutant displayed an obvious dwarfism phenotype, which could be dramatically rescued by application of exogenous GA 3 . The average levels of GAs in the dwarf mutant plants were lower than the wild type of rice. The proteomic analysis showed a total of 6352 proteins that were quantified, among which 688 differentially expressed proteins were identified as GA-responsive. The biological process classification indicated that metabolic process, cellular process and single-organism process related proteins were the dominant protein groups, and the differentially expressed proteins were largely located in chloroplast, cytoplasm, and nucleus and extracellular. Furthermore, these proteins were mainly involved in cell elongation, signal transduction, photosynthesis, and carbohydrate and protein metabolism. These results suggested that proteins involved in cell elongation, signal transduction and carbohydrate metabolism are regulated by GA in rice leaf-sheath growth.

中文翻译：

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GA介导的水稻叶鞘生长机制：蛋白质组学方法

赤霉素（GAs）介导的水稻伸长生长及其相关蛋白质组学与许多多样化生态条件下的作物生产力有关。缺乏对野生和突变水稻植物响应 GAs 处理的叶鞘伸长所涉及的蛋白质组动力学的精确研究。为了探索 GA 诱导水稻叶鞘伸长的分子机制，我们对野生型水稻和 GA 生物合成缺陷突变体 osko2 进行了比较蛋白质组学分析。该突变体表现出明显的侏儒症表型，可通过应用外源GA 3 显着挽救。矮突变株中GAs的平均水平低于野生型水稻。蛋白质组学分析显示，共有 6352 种蛋白质被定量，其中 688 个差异表达蛋白被鉴定为 GA 响应蛋白。生物过程分类表明代谢过程、细胞过程和单生物过程相关蛋白是优势蛋白组，差异表达蛋白主要位于叶绿体、细胞质、细胞核和细胞外。此外，这些蛋白质主要参与细胞伸长、信号转导、光合作用以及碳水化合物和蛋白质代谢。这些结果表明参与细胞伸长、信号转导和碳水化合物代谢的蛋白质在水稻叶鞘生长过程中受 GA 调节。细胞过程和单生物过程相关蛋白是优势蛋白组，差异表达蛋白主要位于叶绿体、细胞质、细胞核和细胞外。此外，这些蛋白质主要参与细胞伸长、信号转导、光合作用以及碳水化合物和蛋白质代谢。这些结果表明参与细胞伸长、信号转导和碳水化合物代谢的蛋白质在水稻叶鞘生长过程中受 GA 调节。细胞过程和单生物过程相关蛋白是优势蛋白组，差异表达蛋白主要位于叶绿体、细胞质、细胞核和细胞外。此外，这些蛋白质主要参与细胞伸长、信号转导、光合作用以及碳水化合物和蛋白质代谢。这些结果表明参与细胞伸长、信号转导和碳水化合物代谢的蛋白质在水稻叶鞘生长过程中受 GA 调节。