Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A5) carrying mcry1Ab and mcry2Ab genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3–V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall α and β-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera Rahnella, Kaistobacter, Pseudomonas and Arthrobacter were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera Bacillus and Flavobacterium. In addition, the relative abundances of nitrogen fixing microbes i.e. Arthrobacter, Burkholderia and Pseudomonas in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying mcry1Ab and mcry2Ab genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages.

中文翻译：

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不同发育阶段携带 mcry1Ab 和 mcry2Ab 基因的田间种植转基因玉米品系细菌根际生物群落的组装和变化

在过去的几十年里，评估转基因作物对根际细菌群落的影响促进了大量研究。为了进一步解决这一问题，我们设计了研究，以检查携带 mcry1Ab 和 mcry2Ab 基因的转基因抗虫玉米品系 2A-5 (2A5) 及其对照 Zheng58 (Z58) 在不同发育阶段的细菌多样性谱的变化。自然田地条件。通过 Illumina MiSeq 对 16S rDNA 基因 (V3-V4) 高变区的高通量测序显示，整体 α 和 β 多样性没有显着变化。然而，在微生物群落的相对丰度中观察到了一些发育阶段依赖性差异。在拔节阶段，变形菌门和 Rahnella 属、Kaistobacter、与大块土壤和周围土壤相比，假单胞菌和节杆菌在 2A5 和 Z58 的根际土壤中大量富集，同时富含芽孢杆菌属和黄杆菌属的微生物。此外，与 Z58 相比，2A5 根际样品中固氮微生物（即节杆菌、伯克霍尔德菌和假单胞菌）的相对丰度在开花和成熟阶段表现出旺盛，这意味着基因改造可能在一些固氮微生物。我们的研究结果表明，携带 mcry1Ab 和 mcry2Ab 基因的转基因玉米几乎没有影响，而且根际细菌群落的变化是由宿主植物在不同发育阶段造成的。