Today there are increasing trends to search for new feed materials or feed additives possessing health benefits for animals and poultry. The present study was conducted to determine the effects of a Chlorella by-product (CBP) on humoral and cell-mediated immune responses, antioxidant status and intestinal mucosal morphology of broiler chickens. Seventy-two Ross 308 male broilers with an initial average weight of 662.7 g at 22 days of age were divided into 3 treatments with 4 replicates of 6 chicks. Treatment groups were as follows: control with 0% CBP and two CBP receiving groups with 10 and 20 g kg⁻¹ supplementation. Broilers with 10 g kg⁻¹ CBP in the feed showed the highest total antibody and immunoglobulin G (IgG) titers against sheep red blood cells as well as higher cutaneous hypersensitivity response as compared with other groups. Increase in serum superoxide dismutase activity and decrease in malondialdehyde concentration were observed in birds with 20 g kg⁻¹ CBP. Villus height and crypt depth were increased significantly by 10 g kg⁻¹ CBP supplement. Taken together, the results of the current experiment indicated that promotion of immune function, improved antioxidant status, and enhancement of intestinal mucosal morphology could be achieved by supplementation of the diet with CBP. Hence, CBP has the potential to be used as a valuable feed supplement in particular at 10 g kg⁻¹ to improve general health of broilers.

如今，寻找对动物和家禽具有健康益处的新型饲料原料或饲料添加剂的趋势不断增加。进行本研究是为了确定小球藻副产物（CBP）对肉鸡体液和细胞介导的免疫反应，抗氧化剂状态和肠粘膜形态的影响。将22日龄时平均平均体重为662.7 g的72头Ross 308雄性肉鸡分为3种处理，每组6只雏鸡进行4次重复。治疗组如下：对照组用0％CBP和两个CBP接受组补充10和20g kg ^-1。10 g kg ^-1的肉鸡与其他组相比，饲料中的CBP显示出针对绵羊红细胞的最高总抗体和免疫球蛋白G（IgG）滴度以及更高的皮肤超敏反应。在20 g kg ^-1 CBP的家禽中观察到血清超氧化物歧化酶活性增加，丙二醛浓度降低。10 g kg ^-1 CBP补充剂可显着增加绒毛高度和隐窝深度。综上所述，当前实验的结果表明，通过在日粮中添加CBP可以提高免疫功能，改善抗氧化状态并增强肠粘膜形态。因此，CBP有潜力用作有价值的饲料补充剂，尤其是在10 g kg ^{-1的情况下} 改善肉鸡的整体健康状况。