Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems need to be solved to make them a viable option. Pre-treatment is necessary to enhance accessibility and solubility of the biomass components but should be minimal and mild to assure sustainable and cost-effective processing. Here, two routes to pre-treatLaminaria digitata to release polysaccharides were investigated: hot water pre-treatment by autoclaving (121 °C, 20 min or 60 min) and a two-step extraction with mild acid (0.1 M HCl) followed by alkaline treatment. Hot water pre-treatment resulted in partial extraction of a mixture of polysaccharides consisting of alginate, fucoidan and laminarin. After mild acid pre-treatment, alginate was found in the remaining insoluble residues and was extracted in a second step via alkaline treatment using Na₂CO₃ (0.15 M) at 80 °C and CaCl₂ (10%) for the precipitation. In addition to carbohydrates, a fraction of other components such as proteins, phenolic compounds, minerals and trace elements was detected in the extracts. Cultivation of the thermophilic bacterial strains Rhodothermus marinus DSM 16675 and Bacillus methanolicus MGA3 (ATCC 53907) in media supplemented with the respective extracts resulted in growth of both strains, indicating that they were able to utilize the available carbon source for growth. R. marinus displayed the highest cell density in the medium containing the extract from acid pre-treatment, whereas B. methanolicus growth was highest with the extract from hot water pre-treatment.

褐藻（Phaeophyta）由于具有高的生物量生产率和碳水化合物含量，具有作为生物精炼厂原料的高潜力。然而，它们是用于微生物精制的具有挑战性的，非常规的原料，需要解决一些加工问题以使其成为可行的选择。预处理对于增强生物质组分的可及性和溶解度是必需的，但预处理应尽量少且温和，以确保可持续且具有成本效益的处理。在这里，有两条路线可以治疗数字海带研究了释放多糖的方法：通过高压灭菌（121°C，20分钟或60分钟）对热水进行预处理，并用弱酸（0.1 M HCl）进行两步萃取，然后进行碱处理。热水预处理导致部分提取包含藻酸盐，岩藻依聚糖和层粘连蛋白的多糖混合物。温和的酸预处理后，在残留的不溶残留物中发现藻酸盐，并在第二步中通过碱处理将其萃取，使用80°C的Na ₂ CO ₃（0.15 M）和CaCl ₂（10％）进行沉淀。除碳水化合物外，提取物中还检测出一部分其他成分，例如蛋白质，酚类化合物，矿物质和微量元素。嗜热细菌菌株的培养在补充有各自提取物的培养基中，海生罗得藻（Rhoderothermus marinus） DSM 16675和甲醇芽孢杆菌MGA3（ATCC 53907）导致两种菌株的生长，表明它们能够利用可用的碳源进行生长。在含有酸预处理提取物的培养基中，R。marinus显示出最高的细胞密度，而热水预处理提取物对甲醇芽孢杆菌的生长最高。