Products derived from wild-grown plants represent a growing segment of the modern pharmaceutical industry. In recent years, particular attention has been paid to their application in the cosmetic industry. The content of B group vitamins in these products is important because they affect the quality of the skin, hair and nails. The determination of thiamine and riboflavin in natural products is not so simple, as it requires complex sample preparation procedures and very sensitive analytical methods. In this paper, the HPLC method with fluorescence detection was applied to the determination of riboflavin and thiamine in the aqueous, ethanol and methanol extracts of elderberry, rosehip, rowanberry, wild garlic and blackthorn. Also, the influence of the extraction solvents on the vitamin’s concentration in obtained extracts was examined. It was found that the content of these vitamins in aqueous extracts is higher than that in alcoholic extracts. Riboflavin concentrations were in the range of 0.84–20.4 μg/g of the dry extract, while thiamine levels were from 0.06 to 0.67 μg/g of the dry extract. The highest riboflavin concentration was found in wild garlic extract followed by rosehip and elderberry extracts. On the other hand, thiamine content was higher in elderberry extract, while in rosehip it was not detected. In this work, the content of riboflavin and thiamine was determined for the first time in extracts of wild garlic, elderberry and rowanberry.

中文翻译：

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药用和化妆品用野生植物提取物中的核黄素和硫胺素含量

来自野生植物的产品代表了现代制药行业的一个增长中的部分。近年来，已经特别注意了它们在化妆品工业中的应用。这些产品中B组维生素的含量很重要，因为它们会影响皮肤，头发和指甲的质量。天然产物中硫胺素和核黄素的测定并不那么简单，因为它需要复杂的样品制备程序和非常灵敏的分析方法。本文采用荧光检测的高效液相色谱法测定接骨木，玫瑰果，罗文莓，野蒜和黑刺李的水，乙醇和甲醇提取物中的核黄素和硫胺素。此外，检查了提取溶剂对所得提取物中维生素浓度的影响。发现水提取物中这些维生素的含量高于酒精提取物中的维生素。核黄素浓度在干提取物的0.84–20.4μg/ g范围内，而硫胺素水平在干提取物的0.06至0.67μg/ g的范围内。在野生大蒜提取物中发现了最高的核黄素浓度，其次是玫瑰果和接骨木浆果提取物。另一方面，接骨木浆果提取物中的硫胺素含量较高，而玫瑰果中未检测到。在这项工作中，首次确定了野生大蒜，接骨木浆果和花an莓提取物中核黄素和硫胺素的含量。67μg/ g干提取物。在野生大蒜提取物中发现了最高的核黄素浓度，其次是玫瑰果和接骨木浆果提取物。另一方面，接骨木浆果提取物中的硫胺素含量较高，而玫瑰果中未检测到。在这项工作中，首次确定了野生大蒜，接骨木浆果和花an莓提取物中核黄素和硫胺素的含量。67μg/ g干提取物。在野生大蒜提取物中发现了最高的核黄素浓度，其次是玫瑰果和接骨木浆果提取物。另一方面，接骨木浆果提取物中的硫胺素含量较高，而玫瑰果中未检测到。在这项工作中，首次确定了野生大蒜，接骨木浆果和花an莓提取物中核黄素和硫胺素的含量。