In this study, we focused on the interference of methylparaben (MP), as the most commonly used paraben, on hemoglobin (Hb) fructation, generating reactive oxygen species (ROS) and resulting lipid peroxidation (LPO). We showed the enhancement of ROS generation and heme degradation during hemoglobin fructation in the presence of MP using some biophysical techniques such as chemiluminescence and fluorescence spectroscopies, respectively. Lipid peroxidation was observed using thiobarbituric acid reactive substances (TBARS) assay, which correlated with ROS production. Also, the results showed that by increasing the levels of fructation, especially in diabetic-like groups, ROS production was increased augmenting the intensity of LPO. This was accompanied by the elevation of malondialdehyde (MDA) production. Thus, MP has an adverse impact on hemoglobin by enhancing advance glycation end products (AGEs) and ROS generation upon its fructation, which results in MDA generation through enhanced LPO. This kind of research emphasises the importance of preservatives’ application revision especially methylparaben in various industries and the necessity of special attention to the consumption of these products by diabetic patients.

中文翻译：

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血红蛋白破碎过程中ROS生成和LPO过程与对羟基苯甲酸甲酯浓度的关系

在这项研究中，我们集中于对羟基苯甲酸甲酯（MP）（最常用的对羟基苯甲酸酯）对血红蛋白（Hb）的干扰，产生活性氧（ROS）并导致脂质过氧化（LPO）的干扰。我们分别使用化学发光和荧光光谱等一些生物物理技术显示了在MP存在下血红蛋白结节过程中ROS生成和血红素降解的增强。使用硫代巴比妥酸反应性物质（TBARS）分析观察到脂质过氧化，这与ROS的产生有关。而且，结果表明，通过增加果糖水平，特别是在糖尿病样组中，ROS产生增加，从而增加了LPO的强度。这伴随着丙二醛（MDA）产量的增加。从而，MP通过增强果糖提前糖基化终产物（AGEs）和ROS生成对血红蛋白产生不利影响，从而通过增强LPO生成MDA。这种研究强调了防腐剂的应用修订的重要性，尤其是对尼泊金甲酯在各个行业的应用，以及糖尿病患者对这些产品的消费的特别关注。