A sensitive method was developed and validated for the determination of UV filters (oxybenzone, octocrylene, 4-methyl-benzylidene camphor, 2-ethylhexyl-4-methoxycinnamate and avobenzone) in tilapia muscle using matrix solid-phase dispersion followed by on-line solid-phase extraction–liquid chromatography/UV. The validation showed good linearity from 100 to 3600 ng g −1 with r 2 values > 0.98. Precision values with relative standard deviations were < 24% for all analytes. The accuracy was evaluated with the recovery of spiked muscle samples, and it ranged between 89 and 96% for most analytes, except 2-ethylhexyl-4-methoxycinnamate. A positive matrix effect was observed for oxybenzone, so a matrix-matched calibration curve was used for quantitation purposes. This methodology was applied to incurred samples of fish exposed to the analytes for 3 days (100 μg L −1 each). Residual quantities of the contaminants were measured after 24, 48 and 72 h of exposure, and concentrations ranged between 400 and 2900 ng g −1 . The developed method was able to quantify all of the target compounds at trace levels.

中文翻译：

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使用离线 MSPD 和在线 SPE-LC/UV 分析罗非鱼中紫外线过滤剂的方法

开发并验证了一种灵敏的方法，用于使用基质固相分散和在线固体测定罗非鱼肌肉中的紫外线过滤剂（氧苯酮、奥克立林、4-甲基-亚苄基樟脑、2-乙基己基-4-甲氧基肉桂酸酯和阿伏苯宗）相萃取-液相色谱/UV。验证显示从 100 到 3600 ng g -1 具有良好的线性，r 2 值 > 0.98。对于所有分析物，具有相对标准偏差的精密度值 < 24%。准确度通过加标肌肉样品的回收率进行评估，除 2-乙基己基-4-甲氧基肉桂酸酯外，大多数分析物的准确度在 89% 到 96% 之间。观察到氧苯酮的基质正效应，因此使用基质匹配校准曲线进行定量。该方法适用于暴露于分析物 3 天的鱼的样本（每个 100 μg L -1）。在暴露 24、48 和 72 小时后测量污染物的残留量，浓度范围在 400 和 2900 ng g -1 之间。开发的方法能够定量所有痕量水平的目标化合物。