Essential and nonessential element concentrations in human blood provide important information on the nutritional status of individuals and can assist in the screening or diagnosis of certain disorders and their association with other causative factors. A simple and sensitive method, suitable for use with small sample volumes, for quantification of multiple trace element concentrations in whole blood and plasma has been developed using inductively coupled plasma-mass spectrometry. Method validation was performed using standard reference materials of whole blood and serum using varying sample treatments with nitric acid, water and hydrogen peroxide. The method was applied to quantify the trace element concentrations in whole blood and plasma samples (0.1 mL) from 50 adult blood donors in Queensland. The whole blood sample (5 mL) was collected in Vacutainer tubes with K₂EDTA as anticoagulant. The developed method was able to quantify, in blood and plasma samples over a wide range of concentrations, several essential elements: cobalt, copper, zinc, iron, manganese and selenium; the nutritionally probably essential elements vanadium and strontium; and nonessential elements including lead, cadmium, arsenic, caesium, barium, thallium and uranium. Significant differences (P < 0.0001) were observed between whole blood and plasma concentrations for 13 elements; 5 of the measured elements, cobalt (0.49 vs. 0.36 μg/L), copper (1.0 vs. 0.75 mg/L), strontium (28 vs. 16 μg/L), barium (1.5 vs. 0.64 μg/L) and thallium (0.06 vs. 0.03 μg/L), had higher mean concentrations in plasma than in blood. Whole blood concentrations of nine trace elements were significantly correlated (P < 0.0001) with plasma concentrations. The distribution of the trace elements between human blood and plasma varied considerably for the different elements. These results indicate that, using a small sample volume, this assay is suitable for the evaluation of nutritional status as well as in monitoring human toxic elemental exposures.

中文翻译：

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ICP-MS方法的开发和验证及其在测定小体积全血和血浆中多种微量元素中的应用

人血中必需和非必需元素的浓度可提供有关个体营养状况的重要信息，并可帮助筛查或诊断某些疾病以及将其与其他原因相关联。使用电感耦合等离子体质谱法已开发出一种适用于小样本量的简单而灵敏的方法，用于定量全血和血浆中多种微量元素的浓度。方法的验证是使用全血和血清的标准参考物质，并用硝酸，水和过氧化氢进行不同的样品处理来进行的。该方法用于量化昆士兰州50位成人献血者的全血和血浆样品（0.1 mL）中的微量元素浓度。₂ EDTA作为抗凝剂。所开发的方法能够在多种浓度的血液和血浆样品中对几种必需元素进行定量：钴，铜，锌，铁，锰和硒。营养上可能必需的元素钒和锶；和非必需元素，包括铅，镉，砷，铯，钡，th和铀。 在全血和血浆中13种元素的浓度之间存在显着差异（P <0.0001）。5种被测元素，钴（0.49 vs.0.36μg/ L），铜（1.0 vs.0.75 mg / L），锶（28 vs.16μg/ L），钡（1.5 vs.0.64μg/ L）和plasma（0.06 vs. 0.03μg/ L）在血浆中的平均浓度高于在血液中的平均浓度。全血中9种微量元素的浓度显着相关（P  <0.0001）与血浆浓度。微量元素在人体血液和血浆之间的分布因不同元素而有很大差异。这些结果表明，使用少量样品，该测定法适合评估营养状况以及监测人体有毒元素的暴露。