Abstract Context: Sanguisorba officinalis L. (Rosaceae), a famous traditional Chinese medicine. It was recently reported that its polysaccharide could facilitate collagen production. Objectives: We investigated the mechanism by which S. officinalis polysaccharide (SOWPa) and/or platelet-rich plasma (PRP) promote regenerative potential of anterior cruciate ligament (ACL) in vitro. Materials and methods: ACL fibroblasts were treated with SOWPa (25 and 100 mg/kg), PRP, PRP + SOWPa (25 and 100 mg/kg) or vehicle alone for 24, 48, or 72 h. Cell viability, migration ability and apoptosis were evaluated by MTT, transwell and flow cytometry, respectively. Western blot analysis was performed to assess associated protein expression. Results: PRP, SOWPa (100 mg/kg) or PRP + SOWPa (100 mg/kg) treatment for 72 h significantly improved the cell viability of ACL fibroblasts from 100 ± 7.5% (control) to 156.85 ± 12.82%, 188.08 ± 15.92%, and 223.67 ± 18.82%, respectively, which was evidenced by individual decreased apoptosis rate from 31.26 ± 2.35% (control) to 20.80 ± 1.89%, 18.01 ± 1.55% and 9.33 ± 0.78%. Furthermore, the motility of ACL fibroblasts was significantly improved with increased migrated cell number per field from 5 for control to 26 for PRP, 36 for SOWPa and 44 for PRP + SOWPa, respectively. Moreover, the protein expression of differentiation markers (RUNX2, ALP, BMP2 and Col I) and TLR-4 and phosphorylated p65 (p-p65) was inhibited by the above treatment. Discussion and conclusions: Data suggested that the addition of SOWPa to PRP increased the regenerative ability of ACL fibroblasts by blocking the TLR-4/NF-κB pathway.

中文翻译：

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富血小板血浆 (PRP) 和山药多糖对体外前交叉韧带 (ACL) 成纤维细胞增殖和分化的积极影响

摘要 背景： 蔷薇科（蔷薇科）名贵中药材。最近有报道称其多糖可以促进胶原蛋白的产生。目的：我们研究了厚朴多糖 (SOWPa) 和/或富含血小板的血浆 (PRP) 在体外促进前交叉韧带 (ACL) 再生潜力的机制。材料和方法：ACL 成纤维细胞用 SOWPa（25 和 100 毫克/千克）、PRP、PRP + SOWPa（25 和 100 毫克/千克）或单独的载体处理 24、48 或 72 小时。分别通过MTT、transwell和流式细胞术评估细胞活力、迁移能力和凋亡。进行蛋白质印迹分析以评估相关的蛋白质表达。结果：PRP，SOWPa (100 mg/kg) 或 PRP + SOWPa (100 mg/kg) 处理 72 小时显着提高了 ACL 成纤维细胞的细胞活力，从 100 ± 7.5%（对照）提高到 156.85 ± 12.82%、188.08 ± 15.92% 和 223.6.分别为 ± 18.82%，这可以通过个体细胞凋亡率从 31.26 ± 2.35%（对照）降低到 20.80 ± 1.89%、18.01 ± 1.55% 和 9.33 ± 0.78% 来证明。此外，ACL 成纤维细胞的运动性显着提高，每个区域的迁移细胞数从对照的 5 个增加到 PRP 的 26 个、SOWPa 的 36 个和 PRP + SOWPa 的 44 个。此外，分化标志物（RUNX2、ALP、BMP2和Col I）和TLR-4和磷酸化p65（p-p65）的蛋白表达被上述处理抑制。讨论和结论：