Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood. Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites. Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers. Results: Bacterial counts ranged from 7.2 ± 2.8 × 105 at the palate before sleep to 1.3 ± 0.2 × 108 at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 106 at the palate before sleep to 9.2 ± 5.0 × 107 at the back of the tongue after sleep, a difference of 48 times. Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.

中文翻译：

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睡眠前后小儿口腔的微生物丰度动态。

目的：口腔中的微生境在微生物分类上有所不同。但是，尚未完全了解这些微生境中细菌和病毒群落的丰富变化。目的和假设：评估睡眠前和睡眠后口腔中6个微生境中微生物丰度的空间分布和动态。我们假设这些微生物群落的丰度分布在口腔部位之间会有所不同。方法：使用流式细胞仪，在10个健康的小儿睡眠者中，对6种口服微生境进行了计数，计算出细菌和病毒样颗粒（VLP）的丰度。结果：细菌计数范围从睡眠前的上颚的7.2±2.8×105到睡眠后舌后的1.3±0.2×108，相差187倍。VLP范围为1.9±1。入睡前的上颚为0×106，入睡后舌后的为9.2±5.0×107，相差48倍。结论：口腔是一个动态的数字异质环境，在睡眠期间微生物群落可以增加1亿。儿科口腔微生物组的定量补充了分类学多样性信息，以显示生物量如何变化以及时空变化。