Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.

中文翻译：

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基于阻抗的单细胞移液。

许多生物学方法都基于单细胞分离。在单细胞系开发中，金标准涉及通过移液管稀释细胞。此过程很耗时，因为要重复几个星期才能确保克隆性。在这里，我们报告了基于库尔特原理的集成了细胞传感器的一次性移液器吸头的建模，设计和测试。我们使用分析模型调查，测试和讨论设计参数对传感器性能的影响。我们还描述了一种系统，该系统能够使用与传感头耦合的仪器移液器分配单个细胞。最重要的是，该系统允许记录阻抗迹线，以用作单电池隔离的证明。我们用磁珠和细胞评估系统的性能。最后，