Phenols are bioactive substances of great interest because of their involvement in plant physiology, their use in many industrial processes, and their impact on human health. This work aims to summarize the varied approaches to the phenolic analysis of chestnut (bark and wood of trunk and branches, leaves, catkins, burs, and fruit) and to collate the optimal conditions into an easy to follow and execute protocol. Phenolic compounds were extracted by solid–liquid extraction and separated by liquid–liquid extraction. Total phenols content was determined by Folin–Ciocalteu assay, condensed tannins by vanillin assay, and hydrolyzable tannins (gallotannins and ellagitannins) by high-performance liquid chromatography quantification of methyl gallate and ellagic acid following acid methanolysis. The lowest temperature for conservation (–80 ℃), lyophilization, and milling (liquid N₂) were the most effective pretreatments for samples. For quantification of tannins, the use of water clearly reduced the sensitivity of the analysis of condensed tannins, whilst the more efficient degradation capacity of sulfuric acid improved the methanolysis of hydrolyzable tannins. These findings were validated using a range of chestnut tissues, and thus confirm the utility and effectiveness of this easy to implement, cost-effective, and efficient protocol.

苯酚是一种备受关注的生物活性物质，因为它们参与了植物生理，它们在许多工业过程中的使用以及对人体健康的影响。这项工作旨在总结板栗（树干和树枝的树皮和木材，叶子，柳絮，伯斯和水果）的酚醛分析的各种方法，并将最佳条件整理成易于遵循和执行的规程。酚类化合物通过固液萃取法提取，并通过液液萃取法分离。总苯酚含量通过Folin–Ciocalteu测定，通过香草醛测定的浓缩单宁，以及通过高效液相色谱法在酸甲醇分解后对没食子酸甲酯和鞣花酸进行定量的可水解单宁（gallotannins和ellagitannins）来确定。最低保存温度（–80℃），₂）是最有效的样品预处理方法。对于单宁的定量分析，用水明显降低了缩合单宁分析的灵敏度，而更有效的硫酸降解能力则改善了可水解单宁的甲醇分解作用。这些发现通过一系列栗子组织得到了验证，从而证实了这种易于实施，具有成本效益和高效协议的实用性和有效性。