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Electroimmunodetection of cardiac C‐reactive protein for determining myocardial Injury
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-04-10 , DOI: 10.1002/bab.1921
Xiaoya Gan 1 , Tao Gong 2 , Yin Zheng 3, 4 , Subash C B Gopinath 5, 6 , Ketong Zhao 3, 4
Affiliation  

C‐reactive protein (CRP) is an acute phase reactant to be a marker of inflammation and has been correlated with the cardiac injury. An immunoassay was performed using anti‐human CRP antibody on an InterDigitated electrode (IDE) sensor to determine and specify CRP concentration for diagnosing the condition of myocardial inflammation. To promote the detection, gold nanoparticle (GNP) was seeded on the aminated‐IDE surface. Anti‐CRP was hitched on the GNP‐seeded surface and identified the abundance of CRP. The limit of quantification was found as 100 fM, and the higher current response was noticed by increasing CRP concentrations with the sensitivity at 1 pM. Furthermore, CRP‐spiked human serum did not interfere the determination of CRP and increased the current response, indicating suitability for a real‐life sample. Similarly, the control experiments with nonimmune antibody Troponin I are not showing the definite current responses, proving the selective identification of CRP. This method of diagnosing is needful to determine the cardiovascular injury at the right time.

中文翻译:

心脏C反应蛋白的电免疫检测确定心肌损伤

C反应蛋白(CRP)是一种急性反应物,可作为炎症的标志物,并与心脏损伤相关。使用抗人CRP抗体在InterDigitated电极(IDE)传感器上进行了免疫测定,以确定和指定CRP浓度以诊断心肌炎症。为了促进检测,将金纳米颗粒(GNP)接种在胺化的IDE表面上。将抗CRP钩在GNP播种的表面上,并确定CRP的含量。定量极限为100 fM,通过增加CRP浓度(灵敏度为1 pM)注意到更高的电流响应。此外,加标CRP的人血清不会干扰CRP的测定,不会增加电流响应,表明适用于真实样品。相似地,非免疫抗体肌钙蛋白I的对照实验未显示明确的电流反应,证明了对CRP的选择性鉴定。这种诊断方法对于确定在正确的时间发生的心血管损伤很有必要。
更新日期:2020-04-10
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